Towards Spatio-Temporal Control in Optical Trapping.

Using both continuous-wave (CW) and high repetition rate femtosecond lasers, we present stable 3-dimensional trapping of 1μm polystyrene microspheres. We also stably trapped 100nm latex nanoparticles using the femtosecond mode-locked laser at a very low average power where the CW lasers cannot trap, demonstrating the significance of the fleeting temporal existence of the femtosecond pulses. Trapping was visualized through dark-field microscopy as well as through a noise free detection using two-photon fluorescence as a diagnostics tool owing to its intrinsic 3-dimensional resolution. Comparison between a Gaussian versus a flat-top Gaussian beam profile demonstrates the importance of laser spatial mode in optical trapping.