BACKGROUND: The vertebrate retina comprises sensory neurons, the photoreceptors, as well as many other types of neurons and one type of glial cell. These cells are generated by multipotent and restricted retinal progenitor cells (RPCs), which express Notch1. Loss of Notch1 in RPCs late during retinal development results in the overproduction of rod photoreceptors at the expense of interneurons and glia. RESULTS: To examine the molecular underpinnings of this observation, microarray analysis of single retinal cells from wild-type or Notch1 conditional knockout retinas was performed. In situ hybridization was carried out to validate some of the findings. CONCLUSIONS: The majority of Notch1-mutant cells lost expression of known Notch target genes. These cells also had low levels of RPC and cell cycle genes, and robustly up-regulated rod precursor genes. In addition, single wild-type cells, in which cell cycle marker genes were down-regulated, expressed markers of both rod photoreceptors and interneurons.
BACKGROUND: The vertebrate retina comprises sensory neurons, the photoreceptors, as well as many other types of neurons and one type of glial cell. These cells are generated by multipotent and restricted retinal progenitor cells (RPCs), which express Notch1. Loss of Notch1 in RPCs late during retinal development results in the overproduction of rod photoreceptors at the expense of interneurons and glia. RESULTS: To examine the molecular underpinnings of this observation, microarray analysis of single retinal cells from wild-type or Notch1 conditional knockout retinas was performed. In situ hybridization was carried out to validate some of the findings. CONCLUSIONS: The majority of Notch1-mutant cells lost expression of known Notch target genes. These cells also had low levels of RPC and cell cycle genes, and robustly up-regulated rod precursor genes. In addition, single wild-type cells, in which cell cycle marker genes were down-regulated, expressed markers of both rod photoreceptors and interneurons.
Authors: T Satow; S K Bae; T Inoue; C Inoue; G Miyoshi; K Tomita; Y Bessho; N Hashimoto; R Kageyama Journal: J Neurosci Date: 2001-02-15 Impact factor: 6.167
Authors: M Joseph Phillips; Peng Jiang; Sara Howden; Patrick Barney; Jee Min; Nathaniel W York; Li-Fang Chu; Elizabeth E Capowski; Abigail Cash; Shivani Jain; Katherine Barlow; Tasnia Tabassum; Ron Stewart; Bikash R Pattnaik; James A Thomson; David M Gamm Journal: Stem Cells Date: 2017-12-25 Impact factor: 6.277
Authors: Isaac M Chiu; Lee B Barrett; Erika K Williams; David E Strochlic; Seungkyu Lee; Andy D Weyer; Shan Lou; Gregory S Bryman; David P Roberson; Nader Ghasemlou; Cara Piccoli; Ezgi Ahat; Victor Wang; Enrique J Cobos; Cheryl L Stucky; Qiufu Ma; Stephen D Liberles; Clifford J Woolf Journal: Elife Date: 2014-12-19 Impact factor: 8.140
Authors: Issam Aldiri; Itsuki Ajioka; Beisi Xu; Jiakun Zhang; Xiang Chen; Claudia Benavente; David Finkelstein; Dianna Johnson; Jennifer Akiyama; Len A Pennacchio; Michael A Dyer Journal: Development Date: 2015-12-01 Impact factor: 6.868