Literature DB >> 23813376

Quantification of microglial phagocytosis by a flow cytometer-based assay.

Refik Pul1, Kandiyil Prajeeth Chittappen, Martin Stangel.   

Abstract

Microglia represent the largest population of phagocytes in the CNS and have a principal role in immune defense and inflammatory responses in the CNS. Their phagocytic activity can be studied by a variety of techniques, including a flow cytometry-based approach utilizing polystyrene latex beads. The flow cytometry-based microglial phagocytosis assay, which is presented here, offers the advantage of rapid and reliable analysis of thousands of cells in a quantitative fashion.

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Year:  2013        PMID： 23813376     DOI： 10.1007/978-1-62703-520-0_14

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  6 in total

1.  Validation of Flow Cytometry and Magnetic Bead-Based Methods to Enrich CNS Single Cell Suspensions for Quiescent Microglia.

Authors:  T A Volden; C D Reyelts; T A Hoke; J Arikkath; S J Bonasera
Journal:  J Neuroimmune Pharmacol       Date:  2015-08-11       Impact factor: 4.147

2.  A Rigorous Quantitative Approach to Analyzing Phagocytosis Assays.

Authors:  Michael D Caponegro; Kaitlyn Koenig Thompson; Maryam Tayyab; Stella E Tsirka
Journal:  Bio Protoc       Date:  2020-08-05

3.  Functional differences between microglia and monocytes after ischemic stroke.

Authors:  Rodney M Ritzel; Anita R Patel; Jeremy M Grenier; Joshua Crapser; Rajkumar Verma; Evan R Jellison; Louise D McCullough
Journal:  J Neuroinflammation       Date:  2015-05-29       Impact factor: 8.322

4.  Effectors of Th1 and Th17 cells act on astrocytes and augment their neuroinflammatory properties.

Authors:  Chittappen K Prajeeth; Julius Kronisch; Reza Khorooshi; Benjamin Knier; Henrik Toft-Hansen; Viktoria Gudi; Stefan Floess; Jochen Huehn; Trevor Owens; Thomas Korn; Martin Stangel
Journal:  J Neuroinflammation       Date:  2017-10-16       Impact factor: 8.322

5.  Absence of Regulatory T Cells Causes Phenotypic and Functional Switch in Murine Peritoneal Macrophages.

Authors:  Jelena Skuljec; Adan Chari Jirmo; Anika Habener; Steven R Talbot; Refik Pul; Ruth Grychtol; Malik Aydin; Christoph Kleinschnitz; Christine Happle; Gesine Hansen
Journal:  Front Immunol       Date:  2018-10-31       Impact factor: 7.561

6.  In vitro evaluation of physiologically relevant concentrations of teriflunomide on activation and proliferation of primary rodent microglia.

Authors:  Tanja Wostradowski; Chittappen Kandiyil Prajeeth; Viktoria Gudi; Jessica Kronenberg; Sina Witte; Marina Brieskorn; Martin Stangel
Journal:  J Neuroinflammation       Date:  2016-09-22       Impact factor: 8.322
  6 in total

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