Literature DB >> 23813371

Microglial activation: measurement of cytokines by flow cytometry.

Deepak Kumar Kaushik1, Anirban Basu.   

Abstract

Cytokine measurement is a prerequisite to understand the inflammatory state of the body. Quantitative analysis of cytokines by Western blotting and ELISA is a daunting task as these are time-consuming and error-prone protocols. With the advent of flow cytometry, the estimation of cytokines using the classical antigen-antibody reaction has become a popular choice with researchers/clinicians. Here, we describe a protocol for multiple cytokine analysis using flow cytometry.

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Year:  2013        PMID: 23813371     DOI: 10.1007/978-1-62703-520-0_9

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN: 1064-3745


  2 in total

1.  MicroRNA 155 regulates Japanese encephalitis virus-induced inflammatory response by targeting Src homology 2-containing inositol phosphatase 1.

Authors:  Menaka Chanu Thounaojam; Kiran Kundu; Deepak Kumar Kaushik; Shalini Swaroop; Anita Mahadevan; Susarla Krishna Shankar; Anirban Basu
Journal:  J Virol       Date:  2014-02-12       Impact factor: 5.103

2.  HSP60 plays a regulatory role in IL-1β-induced microglial inflammation via TLR4-p38 MAPK axis.

Authors:  Shalini Swaroop; Nabonita Sengupta; Amol Ratnakar Suryawanshi; Yogita K Adlakha; Anirban Basu
Journal:  J Neuroinflammation       Date:  2016-02-02       Impact factor: 8.322

  2 in total

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