Literature DB >> 23813370

Lentiviral transduction of cultured microglia.

Takahiro Masuda1, Makoto Tsuda, Hidetoshi Tozaki-Saitoh, Kazuhide Inoue.   

Abstract

Microglial cells are the resident immune-related glial cells of the central nervous system (CNS) that are crucial for maintaining homeostasis and sensing pathological alterations in the nervous system. To improve our understanding of the biological function of microglia, gene-transfer techniques have been improved and become widely used over the past several years. Here, we describe lentiviral-mediated transduction as a valuable tool for transduction of cultured microglial cells.

Mesh:

Year:  2013        PMID: 23813370     DOI: 10.1007/978-1-62703-520-0_8

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN: 1064-3745


  5 in total

1.  Nanoparticle delivery of CRISPR into the brain rescues a mouse model of fragile X syndrome from exaggerated repetitive behaviours.

Authors:  Bumwhee Lee; Kunwoo Lee; Shree Panda; Rodrigo Gonzales-Rojas; Anthony Chong; Vladislav Bugay; Hyo Min Park; Robert Brenner; Niren Murthy; Hye Young Lee
Journal:  Nat Biomed Eng       Date:  2018-06-25       Impact factor: 25.671

Review 2.  Targeting microglia with lentivirus and AAV: Recent advances and remaining challenges.

Authors:  Margaret E Maes; Gloria Colombo; Rouven Schulz; Sandra Siegert
Journal:  Neurosci Lett       Date:  2019-05-31       Impact factor: 3.197

3.  Procedures for Culturing and Genetically Manipulating Murine Hippocampal Postnatal Neurons.

Authors:  Enora Moutin; Anne-Laure Hemonnot; Vincent Seube; Nathalie Linck; François Rassendren; Julie Perroy; Vincent Compan
Journal:  Front Synaptic Neurosci       Date:  2020-04-30

4.  Transcription factor IRF5 drives P2X4R+-reactive microglia gating neuropathic pain.

Authors:  Takahiro Masuda; Shosuke Iwamoto; Ryohei Yoshinaga; Hidetoshi Tozaki-Saitoh; Akira Nishiyama; Tak W Mak; Tomohiko Tamura; Makoto Tsuda; Kazuhide Inoue
Journal:  Nat Commun       Date:  2014-05-13       Impact factor: 14.919

5.  Effective Knockdown of Gene Expression in Primary Microglia With siRNA and Magnetic Nanoparticles Without Cell Death or Inflammation.

Authors:  Alejandro Carrillo-Jimenez; Mar Puigdellívol; Anna Vilalta; Jose Luis Venero; Guy Charles Brown; Peter StGeorge-Hyslop; Miguel Angel Burguillos
Journal:  Front Cell Neurosci       Date:  2018-09-21       Impact factor: 5.505

  5 in total

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