Rachael P Jackman1, Xutao Deng, Douglas Bolgiano, Garth H Utter, Cathy Schechterly, Mila Lebedeva, Eva Operskalski, Naomi L Luban, Harvey Alter, Michael P Busch, Sherrill J Slichter, Philip J Norris. 1. Blood Systems Research Institute, San Francisco, California; Puget Sound Blood Center, Seattle, Washington; University of California, Davis, California; National Institutes of Health, Bethesda, Maryland; University of Southern California, Los Angeles, California; Children's National Medical Center, Washington, DC; Department of Laboratory Medicine, University of California, San Francisco, California; Department of Medicine, University of California, San Francisco, California; University of Washington School of Medicine, Seattle, Washington.
Abstract
BACKGROUND: Both leukoreduction and ultraviolet (UV) light treatment of blood products have been shown to reduce the incidence of HLA antibody development in recipients, but the impact of these treatments on the magnitude and persistence of the antibody response is less clear. STUDY DESIGN AND METHODS: Longitudinal samples from 319 subjects taken from four different study cohorts were evaluated for HLA antibodies to determine the effects of leukoreduction and UV treatment on HLA antibody generation and persistence. RESULTS: Subjects receiving leukoreduced or UV-treated blood products were less likely to generate Class I HLA antibodies, and those receiving leukoreduced blood were also less likely to generate Class II HLA antibodies. Among those receiving nonleukoreduced blood, 55% developed Class I HLA antibodies and 51% developed Class II HLA antibodies compared with 28% (Class I) and 15% (Class II) for those receiving leukoreduced blood and 36% (Class I) and 54% (Class II) for those receiving UV-treated blood. Among alloimmunized subjects, leukoreduction resulted in a significant twofold reduction in the magnitude of Class I HLA antibodies, and UV treatment resulted in a significant threefold reduction in the magnitude of Class II HLA antibodies. Both treatments resulted in shorter persistence of Class I HLA antibodies. CONCLUSIONS: These data demonstrate that leukoreduction and UV treatment of blood products results not only in a reduction in the incidence of HLA antibody production, but also in lower and more transient HLA antibody levels among sensitized transfusion recipients.
BACKGROUND: Both leukoreduction and ultraviolet (UV) light treatment of blood products have been shown to reduce the incidence of HLA antibody development in recipients, but the impact of these treatments on the magnitude and persistence of the antibody response is less clear. STUDY DESIGN AND METHODS: Longitudinal samples from 319 subjects taken from four different study cohorts were evaluated for HLA antibodies to determine the effects of leukoreduction and UV treatment on HLA antibody generation and persistence. RESULTS: Subjects receiving leukoreduced or UV-treated blood products were less likely to generate Class I HLA antibodies, and those receiving leukoreduced blood were also less likely to generate Class II HLA antibodies. Among those receiving nonleukoreduced blood, 55% developed Class I HLA antibodies and 51% developed Class II HLA antibodies compared with 28% (Class I) and 15% (Class II) for those receiving leukoreduced blood and 36% (Class I) and 54% (Class II) for those receiving UV-treated blood. Among alloimmunized subjects, leukoreduction resulted in a significant twofold reduction in the magnitude of Class I HLA antibodies, and UV treatment resulted in a significant threefold reduction in the magnitude of Class II HLA antibodies. Both treatments resulted in shorter persistence of Class I HLA antibodies. CONCLUSIONS: These data demonstrate that leukoreduction and UV treatment of blood products results not only in a reduction in the incidence of HLA antibody production, but also in lower and more transient HLA antibody levels among sensitized transfusion recipients.
Authors: Eva A Operskalski; James W Mosley; Leslie H Tobler; Eberhard W Fiebig; Marek J Nowicki; Larry T Mimms; James Gallarda; Bruce H Phelps; Michael P Busch Journal: Transfusion Date: 2003-10 Impact factor: 3.157
Authors: Martin Karpinski; Denise Pochinco; Iga Dembinski; Willie Laidlaw; James Zacharias; Peter Nickerson Journal: J Am Soc Nephrol Date: 2004-03 Impact factor: 10.121
Authors: Rachael P Jackman; Marcus O Muench; John W Heitman; Heather C Inglis; Jacqueline P Law; Susanne Marschner; Raymond P Goodrich; Philip J Norris Journal: Transfusion Date: 2013-03-03 Impact factor: 3.157
Authors: T E Gorman; C J Julius; R F Barth; A Ng; M S Kennedy; T W Prior; J Allen; L C Lasky Journal: Am J Clin Pathol Date: 2000-05 Impact factor: 2.493
Authors: R D Aach; W Szmuness; J W Mosley; F B Hollinger; R A Kahn; C E Stevens; V M Edwards; J Werch Journal: N Engl J Med Date: 1981-04-23 Impact factor: 91.245
Authors: Rachael P Jackman; Marcus O Muench; Heather Inglis; John W Heitman; Susanne Marschner; Raymond P Goodrich; Philip J Norris Journal: Transfusion Date: 2016-11-18 Impact factor: 3.157