[The structure and polymorphism of the Pain-1 vacuolar invertase locus in Solanum species].

Nucleotide sequences of Solanum vacuolar invertase Pain-1 gene from 17 representatives of five subgenera Potatoe, Solanum, Leptostemonum, Minon, Brevantherum have been obtained. Structure and polymorphism of Pain-1 have been characterized. The length of analyzed DNA fragment from 3'-end of III exon to 5'-end of V exon, ranged from 603 to 977 bp, with a highly variable III intron, saturated numerous indels and SNPs. In the coding region for 27 Solanum accessions 80 SNPs have been detected. 34 SNPs caused amino acid changes. Several single SNPs and SNP sets are found to be specific for single species or taxonomic groups. Estimated genetic distances and reconstructed phylogenic trees showed concordance with previous classifications of the investigated species and showed the utility of the Pain-1 locus for taxonomic identification and Solanaceae phylogeny.