Visual field characteristics in neuromyelitis optica in absence of and after one episode of optic neuritis.

PURPOSE: Optic neuritis (ON) observed during neuromyelitis optica (NMO) is in most cases very severe and with poor prognosis. This study's objective was to analyze visual field (VF) abnormalities observed in the absence of ON and post-ON episode.
METHODS: Twenty-seven cases of both NMO and multiple sclerosis (MS) were selected. Thorough ophthalmologic exam was performed at least 6 months post-ON attack. The VF was collected using the Humphrey 750 perimeter. We used the central threshold tests 24-2 with FASTPAC strategy. The abnormalities were categorized based on the Optic Neuritis Treatment Trial classification.
RESULTS: After one ON, 40% of the NMO group's eyes showed total VF loss (P = 0.01), 21% showed abnormalities of neurologic aspect, and 27% showed fascicular abnormalities of which 12% were altitudinal. Given the total VF loss, the positive predictive value in favor of an NMO was 92.8% and the negative predictive value was 47.3%.
CONCLUSION: Alterations of the VF during the NMO differ from those observed in the course of the MS. One ON, blinding from the first attack, must call to mind an NMO. The altitudinal deficits point to a vascular mechanism.

Introduction

Neuromyelitis optica (NMO), a rare inflammatory and demyelinating disease, impacts specifically the optic nerves and spinal cord.1,2 This pathology is predominantly found among women (80%), spread worldwide, and has poor prognosis. Many clinical, immunological, and histological characteristics separate NMO from multiple sclerosis (MS), including sparing of the brain at the onset of the disease, frequent association (10%–40%) with autoimmune diseases, diffuse damage of the white and grey matter of the spinal cord, as well as the involvement of functional and vital prognosis in the short term.3

Of unknown etiology, NMO is now considered an autoimmune disease linked to humoral immunity pathology, as recently determined based on the finding of the antibody directed against aquaporin 4 (AQP4-Ab).4 Lesions are characterized by perivascular immunoglobulin (IgM) and complement (C9) deposits, but also by necrosis zones.5

Visual function is severely affected as confirmed by frequent ocular attacks and severity of its degradation.6 A remote optic neuritis (ON), prior to NMO, is often considered idiopathic or due to MS; the most frequent treatment is intravenous corticotherapy with high dosage, which generally has poor results.7 Recently, plasma exchanges have been shown to be very efficient treatments for ON due to NMO, increasing the need to differentiate ON due to NMO as soon as possible from ON due to MS.8–10

The study’s objective was to analyze visual field (VF) abnormalities observed in the absence of ON and post-ON episode as well as to specify their role in early NMO diagnosis.

Methods

Our study was conducted at the University Hospital Center of Fort de France in Martinique, where all patients have been regularly followed since 1985. All patients had no previous history of any of the following ON: familial, infectious, glaucoma related, vascular, compressive, due to general diseases, toxic, or due to deficiency. All patients were human immunodeficiency virus (HIV) and human T-lymphotropic virus 1 (HTLV-1) negative.

Population studied

Twenty-seven NMO cases were selected. The NMO diagnosis was based on Wingerchuk et al’s diagnostic criteria established in 1999 and revised in 2006.11,12 The revised diagnostic criteria require the presence of two absolute criteria as well as of two out of three major criteria. The absolute criteria are: the existence of unified or bilateral ON, and of acute myelitis. The major criteria are: normal magnetic resonance imaging (MRI) at the onset of the disease, hypersignal extended to at least three vertebral segments on the medullar MRI, and positive search results for AQP4-Ab. The acute myelitis diagnosis was based on the presence of medullar damage including sphincter, sensitive, or motor disorders, for which maximum deficit is reached in less than 4 weeks. Ocular or medullar attack was defined as the apparition or worsening of signs or symptoms for at least 24 hours. A second attack was a new event that happened at least 1 month after the initial attack. The final index attack confirmed the diagnosis. We revealed that the optic-medullary period matched the time frame that separated the first and final index attack. The disease’s global handicap was ranked using the Kürtzke scale from 0 to 10 (expanded disability status scale [EDSS]).13 We calculated the handicap progression rapport as followed: EDSS/length of the disease (years).

We compared the results of the NMO patients with those obtained in the MS patients’ group matched by age and sex. The MS patients (n = 27) met McDonald et al’s diagnosis criteria.14

We used our Martinique NMO and MS patients’ database to obtain characteristics of the disease’s history. Data was collected prospectively over a 20-year period. All patients were tested for AQP4-Ab.

Analysis

For each patient, a thorough eye exam was conducted at least 6 months post-ON onset, including a precise measure of refraction. Visual acuity was collected using the Snellen and Early Treatment Diabetic Retinopathy Study (ETDRS) scales. Sensibility to spatial contrast was measured with the Pelli-Robson and Sloan charts.15 On the Pelli-Robson test, letters are organized by groups of triplets of different contrast.11 An identified triplet consists of two out of the three letters being correctly recognized. A score of 15 or higher (converted in logarithmic units for statistical analysis) is considered normal. According to Sloan, weak contrast cards depend on the identification of grey letters, whose size progressively decreases. The format is similar to the ETDRS scale, and has five letters per line. These letters appear on a backlit white base (Precision Vision, La Salle, IL, USA) and are tested at 2 m. We used two levels of contrast (1.25% and 2.5%). Each letter read correctly contributed one point to the total score. The total score was the addition of all correctly read letters (0–70). We assessed colors vision with the 100-hue test of Farnsworth. The square root of the score was used for statistical analysis. The score’s normal value by age was obtained using Verriest’s tables. We also used frequency doubling technology perimetry (FDTP) (Carl Zeiss Meditec AG, Jena, Germany). We used the N-30 threshold program. The thickness of retinal peripapillary nerve fibers was measured with a Stratus OCT (Carl Zeiss Meditec AG) equipped with the 4.0 version of the software. We followed the fast retinal nerve fiber layer (RFNL) thickness protocol to collect data. The equipment produced three circular scans (resolution: 256) of 3.4 mm of diameter centered on the head of the optic nerve in 1.92 s. We measured the average thickness of the layer of retinal nerve fiber, as well as the average thickness in the temporal, superior, nasal, and inferior quadrants. The quality of input of selected scans was superior or equal to seven. In the case of a narrow pupil, the OCT was realized after instillation of a drop of tropicamide.

Standard automated perimetry (SAP) was done using the Humphrey Field Analyzer 750 II (Carl Zeiss Meditec AG). We used the test of central threshold 24-2 (54 points tested) with FASTPAC strategy. The VF was considered reliable if false positives, false negatives, and fixation losses were below 33%. A VF was definitely normal if all locations were within normal limits on the total deviation plot. A VF was definitely abnormal if any of the following conditions were met: (1) the Glaucoma Hemifield Test VF index was abnormal; (2) the corrected pattern standard deviation VF index was abnormal (P < 5%); (3) a single point was worse than the 0.5% probability level on the total and/or pattern deviation plot; (4) two clustered points are beyond the reference range (P < 5%), and at least one point was worse than the P < 1% on the total and/or pattern deviation plot (a cluster was defined as ≥two horizontally or vertically, not diagonally, contiguous abnormal points with P < 5%); and/or (5) three or more clustered points were worse than P < 5% on the total and/or pattern deviation plot.

In order to determine the abnormality classification for the entire VF, we used the most predominant defect. The pattern of abnormal points on the deviation plot (total or pattern) showing the greater number of abnormal points, was used to determine the appropriate abnormality classification. When necessary, Goldmann’s VF was also conducted in order to specify the deficit’s form.

The VF abnormalities were ranked in 21 categories based on the Optic Neuritis Treatment Trial (ONTT) classification and reorganized into three principal groups: neurologic, optic nerve, and artifactual abnormalities (Table 1).16

Table 1

Classification of the visual field abnormalities

I. Neurologic abnormalities: chiasmal, retrochiasmal, optic nerve chiasm
I.1. Vertical step	Limited visual field loss that respects the vertical meridian and that includes at least two abnormal points at or outside 15° along the vertical meridian.
I.2. Quadrant	Significant visual field loss throughout an entire quadrant that respects the vertical midline. Essentially all points must have a P < 5% value on the total deviation plot.
I.3. Partial hemianopia	A visual field defect that respects the vertical meridian and that is greater than one quadrant but less than a complete vertical hemifield.
I.4. Hemianopia	A visual field defect that respects the vertical meridian and that involves essentially all points in a vertical hemifield.
I.5. Three quadrant	Significant visual field loss throughout three quadrants; essentially all points must have a P < 5% value on the total deviation plot.
II. Optic nerve abnormalities
II.1. Nerve fiber bundle abnormalities
II.1.a. Temporal wedge	A small visual field defect that is temporal to the blind spot.
II.1.b. Enlarged blind spot	A visual field abnormality in the nerve fiber bundle region that involves at least two points and is contiguous with the blind spot.
II.1.c. Nasal step	Limited visual field loss adjacent to the nasal horizontal meridian with at least one abnormal point at or outside 15° on the meridian. Cannot include more than one significant point (on either plot) in the nerve fiber bundle region on the temporal side.
II.1.d. Paracentral	A relatively small visual field abnormality in the nerve fiber bundle region that is generally not contiguous with the blind spot or the nasal meridian. In particular, it does not involve points outside 15° that are adjacent to the nasal meridian.
II.1.e. Partial arcuate	Visual field loss in the nerve fiber bundle region that extends incompletely from the blind spot to the nasal meridian. The defect is generally contiguous with either the blind spot or the nasal meridian and must include at least one abnormal location in the temporal visual field.
II.1.f. Arcuate	Significant visual field loss in the nerve fiber bundle region, extending across contiguous abnormal points from the blind spot to at least one point outside 15° adjacent to the nasal meridian.
II.1.g. Altidudinal	Severe visual field loss throughout the entire superior or inferior hemifield that respects the horizontal midline, with most points in the hemifield having a P < 5% value on the total deviation plot and the entire horizontal midline demonstrating abnormality.
II.2. Diffuse abnormalities
II.2.a. Multiple foci	Visual field loss that includes two or more clusters of abnormal points (P < 5%) located in different areas of the visual field that do not correspond to a particular abnormality pattern. The loss must be present in both the total and pattern deviation plots.
II.2.b. Widespread	Diffuse visual field loss that includes all four quadrants. The Glaucoma Hemifield Test may show a general reduction of sensitivity or the MD must have a P level < 5%. The corrected pattern standard deviation must not have a P level < 5%. Most abnormal points on the total deviation plot are not abnormal on the pattern deviation plot.
II.3. Central abnormalities
II.3.a. Centrocecal	Visual field loss that is in the macular region and contiguous with the blind spot. The foveal threshold must have a P < 5% value. The loss must be symmetrical above and below the midline.
II.3.b. Central	Visual field loss that is predominantly in the macular region. The foveal threshold must have a P < 5% value, and the visual acuity must be impaired. Can be associated with a single hemifield and paired with another defect.
II.4. Severe abnormalities
II.4.a. Total loss of vision	Severe widespread visual field loss (MD ≤−20.00 dB) with visual acuity not totally impaired.
III. Artifactual abnormalities	Superior depression: Two or more abnormal points in the very superior region. Inferior depression: Two or more abnormal points in the very inferior region. Partial peripheral rim: Generally continuous visual field loss outside 15°, but not in all quadrants; must have some curvature. Peripheral rim: Generally continuous visual field loss outside 15° in all four quadrants, usually with no visual field loss inside 15° on either deviation plot. There must be visual field loss temporal to the blind spot.

Abbreviation: MD, mean deviation.

The data was computerized and analyzed in a strictly anonymous manner using MS Excel (Microsoft Corporation, Redmond, WA, USA) and Statview (SAS Institute Inc, Cary, NC, USA). The following tests were used for statistical analysis: Chi-square test for frequency comparisons, Chi-square Yates correction for small populations, and Student’s t-test for average comparisons. This study was approved by the Consulting Committee for the Protection of Individuals of Biomedical Research sponsored by the French Ministry of Health.

Results

Demographic characteristics of the two groups are displayed in Table 2. NMO patients were on average 47 years old and 92% were females. The average age at the NMO onset was 37.1 ± 12.0 years. NMO and MS had been evolving for at least 10 years. The optic-medullary period was 25.3 ± 38.9 months. 55% of the patients were positive to AQP4-Ab. There was no difference between the two groups regarding average age, sex ratio, age of onset, and length of the disease. The number of attacks per patient, number of attacks the first 2 years, and the yearly rate of attacks were all higher in the NMO group.

Table 2

Demographic comparison between the NMO and MS groups

	NMO (n = 27)	MS (n = 27)	P-value
Age (years)	47.5 ± 10.5	44.5 ± 10.1	0.2
Sex ratio F/M (%F)	25/2 (92.6%)	22/5 (81.5%)	0.4
Age at onset (years)	37.1 ± 12	30.7 ± 9.9	0.053
Disease duration (years)	9.9 ± 7.3	11.7 ± 8	0.3
Optic-medullary time elapsed (years)	25.3 ± 38.9	–
AQP4-Ab positive	15 (55.5%)	0
EDSS	5.3 ± 2.5	3.85 ± 2.17	0.1
IP EDSS	0.95 ± 1.51	0.8 ± 1.2	0.2
Ocular presenting symptom	16 (59.2%)	8 (29.6%)	0.055
Number of attacks/patient	1.55 ± 1.8	0.63 ± 1.07	0.0002
Number of attacks/patient in the first 2 years	1.4 ± 0.84	0.55 ± 0.69	0.0002
Annual relapsing rate	0.44 ± 0.8	0.13 ± 0.14	0.0006

Abbreviations: EDSS, expanded disability status scale; F, female; IP, index of progression; M, male; MS, multiple sclerosis; NMO, neuromyelitis optica.

Ten NMO and 30 MS eyes had no previous history of ON. Thirty-three NMO and 19 MS eyes had one previous ON attack. Eight NMO and three MS eyes had two previous attacks. Four NMO and two MS eyes had more than two previous attacks.

The results of the visual function and of optic nerve fibers thickness in absence of and post-ON episode are presented in Table 3. For the eyes with no ON history, there was no difference in visual acuity (Snellen) and optic nerve fibers thickness between the two groups. Nevertheless, ETDRS visual acuity, contrasts vision (Sloan chart 1.25%), mean deviation SAP, and foveal threshold were not as good in the MS group.

Table 3

Visual function and optic nerve fibers thickness in the absence of prior history and after an optic neuritis episode

Number of optic neuritis episodes	0			1
	NMO (n = 10 eyes)	MS (n = 30 eyes)	P-value	NMO (n = 33 eyes)	MS (n = 19 eyes)	P-value
Visual acuity (Snellen)	20/20	20/20	0.4	20/50	20/30	0.02
Visual acuity ETDRS	61.1 ± 4.6	55.6 ± 5.6	0.006	31.4 ± 24.6	44.1 ± 18.6	0.04
Visual acuity ≤20/200	0	0	–	14 (42.4%)	4 (21%)	0.03
Pelli–Robson chart	1.7 ± 0.4	1.6 ± 0.1	0.08	0.93 ± 0.7	1.2 ± 0.5	0.09
Sloan chart 1.25%	20.9 ± 7.1	12.1 ± 9.5	0.02	3 ± 5.9	6 ± 7.9	0.3
Sloan chart 2.5%	30.4 ± 11.2	26.2 ± 9.9	0.06	9.3 ± 13.3	15 ± 14	0.1
MD (SAP)	−1.8 ± 0.9	−3.7 ± 3.1	0.01	−10.3 ± 10	−7.9 ± 5.7	0.9
PSD (SAP)	2 ± 0.4	2.9 ± 1.4	0.007	5 ± 3.1	4.8 ± 2.5	0.9
Foveal threshold	34.4 ± 1.8	32.5 ± 3.1	0.07	16.2 ± 15.2	27.4 ± 10.4	0.01
MD (FDTP)	−0.5 ± 3.3	−3.1 ± 3.8	0.09	−6.2 ± 5.9	−3.4 ± 3.6	0.1
PSD (FDTP)	3.5 ± 0.4	5.6 ± 2.8	0.01	6.7 ± 3.5	6.2 ± 3.3	0.3
100-Hue	43.7 ± 53.4	74.1 ± 91	0.4	91.4 ± 91.2	135.5 ± 142.3	0.3
Overall average RNFL thickness (μm)	95.6 ± 12.1	96.3 ± 13	0.5	64.4 ± 22.8	77 ± 21.2	0.06

Abbreviations: ETDRS, Early Treatment Diabetic Retinopathy Study; FDTP, frequency doubling technology perimetry; MD, mean deviation; MS, multiple sclerosis; NMO, neuromyelitis optica; PSD, pattern standard deviation; RNFL, retinal nerve fiber layer; SAP, standard automated perimetry.

After one ON attack, Snellen visual acuity, ETDRS, and the optic nerve fibers thickness were smaller in the NMO group than in the MS group (20/50 versus 20/30, P = 0.02; 31.4 versus 44.1, P = 0.04; 64.4 μm versus 77 μm, P = 0.06, respectively). The proportion of eyes with a visual acuity ≤20/200 was 42% in the NMO group versus 21% in the MS group (P = 0.03).

Table 4 shows the results of the VF morphological analysis in absence of and post-ON episode. In the absence of ON, the foveal threshold was 34 for the NMO group and 32.5 for the MS group (P = 0.07). Half of the NMO patients’ VFs were normal, a third of the MS patients’ VFs were normal, and one third of the MS patients’ VFs showed diffuse lesions. In the absence of ON there was no difference in the distribution of VF deficits between the two groups.

Table 4

Visual field abnormalities in absence of and after one optic neuritis episode

Number of optic neuritis episodes	0			1
	NMO (n = 10 eyes)	MS (n = 30 eyes)	P-value	NMO (n = 33 eyes)	MS (n = 19 eyes)	P-value
Foveal threshold	34 ± 1.8	32.5 ± 3.1	0.07	16.2 ± 15.2	27.4 ± 10.4	0.01
I. Neurologic abnormalities	0	1 (3.3%)	0.9	7 (21.3%)	3 (15.8%)	0.9
I.1. Vertical step	0	0	–	0	0	–
I.2. Quadrant	0	0	–	3 (9%)	0	0.4
I.3. Partial hemianopia	0	0	–	1 (3%)	0	0.9
I.4. Hemianopia	0	0	–	1 (3%)	0	0.9
I.5. Three quadrant	0	1 (3.3%)	0.9	2 (6%)	3 (15.8%)	0.5
II.1. Nerve fiber bundle abnormalities	3 (30%)	8 (26.7%)	0.9	9 (27.3%)	4 (21%)	0.9
II.1.a. Temporal wedge	0	0	–	0	0	–
II.1.b. Enlarged blind spot	0	0	–	0	1 (5.3%)	0.9
II.1.c. Nasal step	0	3 (10%)	0.7	1 (3%)	0	0.9
II.1.d. Paracentral	3 (30%)	4 (13.3%)	0.4	3 (9%)	0	0.4
II.1.e. Partial arcuate	0	0	–	0	1 (5.3%)	0.9
II.1.f. Arcuate	0	1 (3.3%)	0.9	1 (3%)	2 (10.5%)	0.6
II.1.g. Altidudinal	0	0	–	4 (12.1%)	0	0.3
II.2. Diffuse abnormalities	2 (20%)	9 (30%)	0.8	2 (6%)	8 (42.1%)	0.005
II.2.a. Multiple foci	2 (20%)	4 (13.3%)	0.9	0	2 (10.5%)	0.2
II.2.b. Widespread	0	5 (16.6%)	0.4	2 (6%)	6 (31.6%)	0.04
II.3. Central abnormalities	0	2 (6.7%)	0.9	0	2 (10.5%)	0.2
II.3.a. Centrocecal	0	0	–	0	1 (5.3%)	0.9
II.3.b. Central	0	2 (6.7%)	0.9	0	1 (5.3%)	0.9
II.4.a. Total loss of vision	0	0	–	13 (39.4%)	1 (5.3%)	0.01
Artifactual abnormalities	0	0	–	0	0	–
Normal	5 (50%)	10 (33.3%)	0.5	2 (6%)	1 (5.3%)	0.9

Abbreviations: MS, multiple sclerosis; NMO, neuromyelitis optica.

Post-ON attack, the foveal threshold was 16.2 in the NMO group and 27.4 in the MS group (P = 0.01), and almost 40% of the NMO group’s eyes showed total VF loss versus only 5.3% for MS group’s eyes (P = 0.01). In the case of total VF loss, the positive predictive value in favor of NMO was 92.8% and the negative predictive value was 47.3%. In the NMO group, neurological abnormalities were found in 21.3% of cases and fascicular abnormalities in 27.3%, of which 12.1% were altitudinal deficits (Figures 1 and 2). No altitudinal deficit was observed in the MS group. Diffuse abnormalities were found in 42.1% of the MS eyes versus 6% of the NMO eyes (P = 0.005). In the presence of a diffuse deficit, the positive predictive value in favor of MS was 80% and the negative predictive value 73.8%.

Figure 1

Standard automated perimetry.

Note: Left eye: altitudinal deficit.

Abbrevations: PSD, pattern standard deviation; MD, mean deviation; HFA, Humphrey Field Analyzer; DOB, date of birth; GHT, glaucoma hemifield test; RX, refraction; DC, power of the cylinder; Pos, positive; Neg, negative.

Figure 2

Goldmann’s visual field.

Note: Left eye: altitudinal deficit.

Discussion

Our study showed that after a first ON attack, 40% of the NMO patients’ eyes presented total VF loss and that the ON complicated with total VF loss was, over nine out of ten times, an NMO. In Fernandes et al’s study, after a single ON episode, a mean deviation lower than −20 dB was associated with a 6.0 odds ratio for NMO.17 In two out of three cases in another study, NMO started with ON, sometimes bilaterally.6 It was rare to see the ON associated with myelitis or appearing after the discovery of medullar lesions. Lennon et al3 identified a biological marker (AQP4-Ab) to differentiate NMO from MS. However, this antibody directed against aquaporin-4 has a sensitivity of 60%–70% and specificity of 91%.4,18 Further, in many cases, MRI of isolated ON is normal; ON is then considered idiopathic or due to MS. In contrast with the idiopathic or MS-related ON for which vision is usually recovered in a few weeks, ON due to NMO has a very poor prognosis.19

Thus, the relation to MS or NMO must be identified as soon as possible in order to start the proper treatment for the acute phase as well as a thorough immunosuppressant treatment. Besides MS, blinding ONs are exceptional. They only represent 3.6% of observed ON throughout the course of MS, even course though 36.9% of patients show total vision loss at the acute phase.16 In Slamovits et al’s study, among 12 ON cases without light perception, eight recovered with a visual acuity superior to 20/40 and four with a visual acuity less than 20/40 but with periphery vision conservation. None stayed blind.20

A first ON attack complicated by total vision loss in an AQP4-Ab negative patient, female and of African, Indian, or Asian decent must be considered as the first NMO sign. Indeed, the ocular start and ON frequency are more frequent among these populations while the onset and frequency of medullar attacks is higher in the white population.21,22 Optical coherence tomography can also contribute to orient the NMO diagnosis when RNFL is inferior to 50 μm and when mostly superior and inferior quadrants are concerned.23,24

Given the deficit’s seriousness and fast onset of irreversible optic nerve lesions, the treatment of NMO due to ON is an emergency. Because of the good results obtained through plasma exchanges, we propose their systematic use in association with intravenous corticotherapy, rather than waiting to conclude the inefficacy of corticotherapy alone.8,10,25

Localized anomalies (neurologic anomalies and nerve fiber bundle anomalies) concern about 50% of the deficits in our series. Fernandes et al, whose series’ numbers were similar to ours, also found half of the deficits to be localized.17 Nakajima et al collected VFs for 15 NMO patients with Goldmann perimetry.26 He found, as we did, about 25% of deficits with a neurological aspect.26 In Sadda et al’s study, after a posterior ischemic ON, one third of the eyes kept a visual acuity inferior to 20/640 and when measurable, 30% of the VF showed altitudinal deficit.27 The rarity of diffuse lesions (6% in our series), the localized deficits depth, high proportion of neurological aspect deficits, as well as RNFL significant decrease of the four quadrants, show a severe axonal lesion compared to MS. These severe axonal lesions recall those observed throughout the course of vascular related neuropathies such as the ischemic or glaucomatous ON.23,24 In Fernandes et al’s and our series, the proportion of localized deficits among MS patients was smaller, one third, and appropriate for the ONTT results at 1 year.17 Consequently, it is very common throughout the course of MS to observe a selective reach of the interpapillomacular bundle.

Four eyes (12.1%) of NMO patients showed altitudinal deficits. This type of deficit was not found among the 27 eyes of Fernandes et al’s series, but was noted as the most frequent of the non-central deficits in Nakajima et al’s series.17,26 These altitudinal deficits had all been observed at the time of the first ON attack; they were superior in three cases and inferior in two cases. Altitudinal deficit is usually characteristic of ischemic ON linked to an occlusion of the posterior ciliary arteries. Encountered throughout the course of NMO, such a deficit brings to mind a vascular contribution in ON occurrence. Further, throughout the NMO, the optic nerve appears grayish and softened. The demyelinization predominates at the center of the nerve and is sometimes at the origin of a central cavitation.28 As a consequence of the AQP4-Ab presence, they are associated to perivascular immunoglobulin deposits (Ig M) and complement (C9), axonal necrosis lesions, and atrophy that are never observed throughout the MS.29 Other components in favor of the vascular contribution to ON hypothesis include the revelation of retinal vascular anomalies of attenuation type on the peripapillary arterioles and frosty or occluded aspect of the arterioles located at more than two papillary diameters of the papilla.30 All of these vascular anomalies would be the result of an inflammatory reaction caused by the AQP4-Ab directed against aquaporin-4, strongly expressed at the level of the optic nerve astrocytes and the arterioles of the retina’s deep layer.31

Identifying NMO as soon as possible and putting in place the most adapted treatment is critical for a lesion with poor ocular prognosis and for which vital prognosis is very unfavorable. The VF lesions observed after one episode of ON in NMO are different from those usually observed in MS. Given a total VF loss, the positive predictive value in favor of an NMO was 92.8% and the negative predictive value was 47.3%. Obtained with an ambispective study, these values should be confirmed with a prospective study. The VF analysis helps to invoke the NMO diagnosis when faced with severe or altitudinal lesions that appeared during the first ON attack.