An ex vivo study on immunohistochemical localization of MMP-7 and MMP-9 in temporomandibular joint discs with internal derangement.

Internal derangement (ID) is among the most common disorders of the temporomandibular joint (TMJ). Previous research by our group highlighted a correlation between apoptosis and TMJ ID. Metalloproteinases (MMP)-7 and -9 have been shown to play an important role in extracellular matrix ECM) homeostasis and, through it, in joint disc remodelling. The immunohistochemical expression of MMP-7 and -9 was investigated in discs from patients with TMJ ID and from healthy donors and compared with the degree of histological tissue degeneration. The collagen fibre arrangement in pathological discs exhibited varying degrees of disruption. New vessels were consistently detected; endothelial cells from these vessels were immunolabelled with both MMP-7 and MMP-9. More or less intense MMP-7 and MMP-9 immunolabelling was detected in the cytoplasm of disc cells from all patients. MMP-7 and MMP-9 immunostaining was significantly different between pathological and normal discs and correlated with the extent of histopathological degeneration. MMP-7 and MMP-9 upregulation in discs from patients with TMJ ID demonstrates their involvement in disc damage in this disorder. A greater understanding of these processes could help identify ways to curb MMP overproduction without affecting their tissue remodelling action. The design of specific inhibitors for these MMPs would not only help to gain insights into the biological roles of MMPs, but would also aid in developing therapeutic interventions for diseases associated with abnormal ECM degradation.

Introduction

Internal derangement (ID) is among the most common disorders of the temporomandibular joint (TMJ). Derangement, a clinical feature used to classify TMJ disorders (TMDs), is also used to indicate an abnormal relationship of the articular disc to the mandibular condyle and the articular eminence. TMJ disc displacement is followed by progressive degradation of the extracellular matrix (ECM) of its connective tissue and by histopathological changes that eventually lead to permanent loss of function. Joint changes begin to arise in the posterior disc band, based on the type and extent of disc displacement; advanced ID corresponds to a degenerated disc configuration.

Normal TMJ disc contains ECM macromolecules, i.e. collagen, glycosaminoglycans, proteoglycans and other molecules related to joint disc lubrication. The collagen types identified in this tissue to date are I, II, III, VI, IX, and XII. TMJ disc collagen is almost entirely type I with trace amounts of types II and III. In healthy TMJ disc the balance between ECM synthesis and degradation results in a state of dynamic equilibrium. Pathological disc changes can therefore be the consequence of disruptions of this balance favouring proteolysis. The main molecules involved in ECM degradation are metalloproteinases (MPs) and their inhibitors.

Matrix metalloproteinases (MMPs), collectively called matrixins, participate in ECM degradation. In physiological conditions their activities are precisely regulated at the level of transcription, activation of the precursor zymogens, interaction with specific ECM components, and inhibition by endogenous inhibitors. Disruption of this control may result in diseases of the joints as well as of the orofacial region. Animal and human studies both indicate that at least collagenases MMP-1, -8, and -13, gelatinases MMP-2 and -9, stromelysin MMP-3, and matrilysin MMP-7 are involved in different stages of joint disease pathogenesis. Increased concentrations of MMP-1,-2,-3 and -9 have been detected in TMJ synovial fluid of patients with IDs and/or osteoarthritis (OA). Information regarding MMP-9 expression is scarce, at least at the protein level, nor have any data been reported on MMP-7. Exhaustive information on MMP immunohistochemical expression in TMJ disc tissue is available only for MMP-13. MMP-7 (matrilysin 1) processes ECM components as well as cell surface molecules such as pro-α-defensin, Fas-ligand, pro–tumour necrosis factor (TNF)-α, and E-cadherin. MMP-9, a gelatinase (gelatinase B), digests denatured collagens.

MMPs also have a role in cell proliferation, migration, differentiation, and apoptosis and in angiogenesis. ECM degradation by MMP-9 promotes cell death. Previous research by our group has highlighted a correlation between apoptosis and TMJ ID, and an important role for MMP-9 and 7 in ECM homeostasis.

The aim of this immunohistochemical study was to investigate their expression in TMJ discs with ID and compare the immunohistochemical data to the degree of tissue degeneration.

Materials and Methods

Patients and tissues

Twenty-five paraffin blocks of displaced TMJ disc specimens from earlier investigations were retrieved from the archives of the University of Catania (Italy). Their use had already been approved by the relevant ethics committees; similarly, the patients’ informed consent had been obtained before tissue collection. The discs had been removed from 16 females and 9 males with TMJ ID, 11 with anterior disc displacement with reduction (ADDwR) and 14 with anterior disc displacement without reduction (ADDwoR) diagnosed on the basis of history, clinical examination, and magnetic resonance imaging data. Mean patient age at the time of surgery was 34.2±5.4 years; mean disease duration from ID symptom onset to surgery was 8.7±1.2 months. Unassisted maximum mouth opening (MMO) and a visual analogue scale (VAS) for pain were used to assess disease severity. MMO was measured with a millimetre ruler as interincisal distance; pain intensity in the preceding week was rated by patients on a 100 mm VAS from 0 (no pain) to 100 (the worst imaginable pain). The diagnosis that led to disc excision was ID with pain and functional impairment. The inclusion criteria for this study were: i) unsuccessful conservative management; ii) tenderness to TMJ palpation; and iii) TMJ pain or interference with jaw movement; exclusion criteria were: i) other TMJ diseases; ii) dentofacial deformity; iii) major jaw trauma; iv) previous TMJ surgery; and v) prior TMJ treatment with steroid injections. The discs removed from the 25 patients were macroscopically deformed and none had preserved a normal biconcave shape. The anterior, intermediate and posterior band were preserved in all specimens. Four TMJ discs from the collection of Catania University’s Anatomy Institute were also studied. They were autopsy specimens from one male and three female donors (mean age 49.7±4.4 years) which were selected for their virtually normal shape and condition, since none had macroscopic signs of degenerative or inflammatory joint disease on dissection, nor were they displaced; in addition the donors’ clinical histories were negative for generalized joint disease or TMJ arthropathy.

Histology

The TMJ discs were explanted, cleaned of adhering tissues, and fixed in 10% buffered formalin for 24 h. They were processed as described previously. Briefly tissues were dehydrated using the following sequence: 50% EtOH × 1 h, 70% EtOH × 1 h, 80% EtOH × 1.5 h, 95% EtOH × 12 h, 100% EtOH × 1 h-repeat twice, xylene × 1 h, placed in a metal mold and embedded in paraffin at 60°C overnight. Section of 5 µm were cut and stained with haematoxylin for routine morphological evaluation. Disc sections were observed with an Axioplan Zeiss light microscope (Jena, Germany).

Immunohistochemistry

Sections were processed as previously described.[29,30] Briefly, they were incubated for 30 min in 0.3% H2O2/methanol to quench endogenous peroxidase activity then rinsed for 20 min with phosphate-buffered saline (PBS; Bio-Optica, Milan, Italy). The sections were irradiated as previously described. Then, half of the sections of each sample were incubated in rabbit polyclonal anti-MMP-9 (NB-P1-72189) and the other half in anti MMP-7 antibody (NB-300 1000; both from Novus Biologicals, Littleton, CO, USA) diluted 1:100 in PBS, 0.1% BSA, and incubated overnight at 4°C. The secondary antibody, biotinylated anti-mouse/anti-rabbit IgG, was applied for 30 min at room temperature followed by the avidin-biotin-peroxidase complex (Vector Elite Kit Abbott, Chicago, IL, USA). The immunoreaction was visualized by incubating sections in a 0.1% 3,3’-diaminobenzidine and 0.02% hydrogen peroxide solution (DAB substrate kit, Vector Laboratories, Burlingame, CA, USA) for 4 min. Finally sections were lightly counterstained with Mayer’s haematoxylin (Histolab Products AB, Goteborg, Sweden) and mounted on GVA mount (Zymed Laboratories, San Francisco, CA, USA).

MPP-7 and MMP-9 staining intensity and the proportion of immunopositive cells found in each of the three areas of the disc (anterior, intermediate zone and posterior) were examined in blind by light microscopy and recorded by three anatomists. The three disc areas, anterior, intermediate and posterior band, were examined. Intensity of staining (IS) was graded from 0 to 4 as: 0 = no detectable staining, 1 = weak, 2 = moderate, 3 =strong, 4 = very strong. Cells immunopositive for MPP-7 and MMP-9 [extent score (ES)] were expressed as a percentage of the final number of 100 cells at 200x magnification and classed separately by the three anatomists into four categories: 0 = <5%; 1 = 6–30%; 2 = 31–50%; 3 = >50%, and 4 = >75%. The final staining score (FSS) represented the sum of IS and ES. The FSS obtained for each of the 3 disc areas were added and divided by 3 to calculate the mean (± standard deviation) FSS for each disc. Positive and negative controls were performed as previously described. A disc histopathological degeneration score was calculated for each disc as described previously.

Statistical analysis

The expression level of MMP-7 and MMP-9 was compared with the histopathological degeneration score using Spearman’s test. Interobserver agreement was expressed as kappa coefficient. The anterior, intermediate and posterior bands were compared in ADDwR and ADDwoR patients. All data were analysed with the SPSS program (SPSS® release 16.0, Chicago, IL, USA).

Results

Abnormal collagen fibre arrangement and fragmentation and tearing of collagen bundles were demonstrated in patients’ haematoxylinstained discs, whereas multidirectional collagen bundles were preserved in control discs. Patients’ discs also showed reduced cellularity and an altered cell population ratio, with an increased number of chondrocyte-like cells related to the severity of the morphological disc damage, and a decreased number of fibroblast-like cells, particularly in ADDwR discs.

The TMJ ID patients had received a variety of unsuccessful non-surgical treatments for at least 6 months prior to surgery. Histological examination of haematoxylin-stained discs demonstrated in all specimens different degrees of disruption of the collagen fibre arrangement that ranged from fragmentation to tears and splitting. New vessels were always detected. The mean histopathological score of the 25 discs was 4.27±1.76 (range 3-7). Multidirectional collagen bundles were preserved in control discs, which also showed an absence of new vessels and of signs of nonspecific tissue degeneration.

More or less intense MMP-7 and MMP-9 immunolabelling was detected in the cytoplasm of disc cells from all patients (Figures 1, 2 and 3). The mean FSS of the pathological discs was 2.27±0.71 (range 1.6-3.9) for MMP-7 and 2.87±0.92 (range 1.6-3.7) for MMP-9. The control discs exhibited faint immunostaining (MMP-7, 0.65±0.41 and MMP-9, 0.45±0.75). A greater proportion of cells positive for MMP-7 and MMP-9 was documented in ADDwR and ADDwoR sections than in normal disc. Immunopositivity also varied in the different disc areas; in particular, labelled cells were significantly more numerous (P<0.01) in the posterior band compared with the anterior and intermediate bands in ADDwoR discs. In addition, a significantly greater proportion of labelled cells was seen in the anterior (+) and intermediate (++) band of ADDwR compared with ADDwoR discs (both bands, P<0.05). No difference among disc regions was found in normal specimens. MMP-7 was also detected in synovium, chiefly in cells; very small amounts were bound to the ECM, indicating its synthesis. Intracellular MMP-7 staining was focal, with marked regional variations, but the extent of staining generally correlated (P≤0.01) with the degree of synovial inflammation. Endothelial cells from newly-formed vessels were immunolabelled with both MMP-7 and MMP-9. The kappa coefficient was 0.89 (nearly perfect agreement). The difference in MMP-7 and MMP-9 immunostaining between pathological and normal discs was significant (P=001) and correlated with the histopathological degeneration score (Spearman’s rho=0.639 for MMP-7 and =0.529 for MMP-9).

Figure 1.

A) MMP-7 immunoexpression in fibroblast-like and chondrocyte-like cells in the posterior band of the TMJ ID disc from a patient with ADDwoR; newly-formed vessels are also immunolabelled; magnification: 20x; scale bar: 100 µm. B) Higher magnification of the posterior band of the TMJ ID disc from a patient with ADDwoR shows MMP-7 localization in endothelial cells from newly-formed vessels; magnification: 40x; scale bar: 50 µm.

Figure 2.

A) MMP-9 immunostaining in fibroblast-like and chondrocyte-like cells of the posterior band of the TMJ ID disc from a patient with ADDwoR; magnification: 20x; scale bar: 100 µm. B) Higher magnification of the posterior band of the TMJ ID disc from a patient with ADDwoR with chondrocyte-like cells immunolabbeled with MMP-9; magnification: 40x; scale bar: 50 µm.

Figure 3.

MMP-7 immunoexpression in synovium of a patient with TMJ ID and ADDwoR; magnification: 40x; scale bar: 50 µm.

Discussion

Degenerative changes alter the physical and functional properties of the TMJ, leading to an inability to withstand loading stress that eventually becomes irreversible.[33,34] Clinically mild TMJ ID is characterized by disc displacement with or without bone remodelling, whereas severe ID involves disc or disc attachment perforation, bone remodelling, and osteoarthritic changes. The different proportions of cells staining for MMP-7 and MMP-9 documented in the three regions of pathological compared with normal discs are explained by the site where the maximum compressive stress is exerted. Disc displacement during mouth opening entails that in pathological disc this stress is exerted on the posterior band. Moreover different immunolabelling patterns were also found in ADDwR and ADDwoR discs. In the former discs maximum stress is exerted on the anterior band before disc recapture; when the disc is reduced, the maximum stress is exerted on the intermediate zone, where caspase 3-positive cells were indeed most numerous. In ADDwoR discs maximum compressive stress is exerted on the posterior band, where we detected a greater expression of caspase-3 compared with the other two bands. These pathophysiological considerations contribute to explain the finding of different proportions of cells positive for MMP-7 and MMP-9 in posterior, intermediate and anterior disc bands. Different types of collagen and non-collagenous proteins as well as MMPs seem to be involved in disc and joint changes, at least as demonstrated by examination of synovial fluid from patients with TMDs. However, even though immunohistochemical findings provide critical clinical information,[36,37] very little work has been done on TMJ disc MMPs because disc tissue is difficult to obtain.

The present study demonstrates for the first time that MMP-7 and MMP-9 are upregulated in discs from patients with TMJ ID and provides evidence of a role for them in disc damage in this disorder. Interestingly, MMP-7 and MMP-9 are expressed in arthritic joints and can degrade a number of matrix proteins in the joint. Furthermore they are involved in new blood vessel formation through cleaving of plasminogen and generation of angiostatin molecules; these findings may partly account for the newly formed vessels found in pathological TMJ discs. Increased immunoreactivity for elastolytic proteases, particularly MMP-7 and MMP-9, has also been demonstrated in areas of elastin depletion in floppy eyelid syndrome specimens compared with controls. Unexpectedly, elastic system fibres (oxytalan, elaunin, and elastic fibres) have been described in severely damaged TMJ disc, where they appear to ensure biomechanical resistance to stretch and compression by reinforcing regions lacking collagen bundles, thus serving as shock absorbers. However, the findings of the present study suggest that this may be a defence mechanism, since elastic system fibres are degraded by MMPs even as they are synthesized. A greater proportion of MMP-7 and MMP-9-positive cells was detected in the posterior than in the anterior and intermediate bands of ADDwR and ADDwoR discs, demonstrating that disc degeneration begins in this area, as documented by Orhan and colleagues.

The expression of these MMPs is regulated by several factors including a variety of cytokines (e.g. interleukin 1 and TNF-α), which play an important role in TMJ ID pathogenesis and have indeed been demonstrated in synovial fluid of pathological TMJ. In contrast, tissue inhibitors of metalloproteinases (TIMPs) are matrixin inhibitors involved in the local control of MMP activities in tissues. A greater understanding of these processes could identify ways to curb MMP overproduction without compromising tissue remodelling.

The design of specific inhibitors for these MMPs is therefore an important objective. Such inhibitors would not only help to gain insights into the biological roles of MMPs, but they would also aid in developing therapeutic interventions for diseases associated with abnormal ECM degradation, at least to slow down disease progression. Furthermore, MMP inhibitors such as sub–antimicrobial levels of tetracyclines or non–antimicrobial chemically modified tetracyclines could be beneficial in conditions involving MMP-mediated inflammatory tissue destruction, including TMJ ID. The findings of the present study contribute some novel information towards this purpose.