Vandana Rai1, Upendra Yadav, Pradeep Kumar. 1. Department of Biotechnology, Human Molecular Genetics Laboratory, VBS Purvanchal University, Jaunpur, Uttar Pradesh, India.
Abstract
OBJECTIVE: This study was aimed to evaluate the Methionine synthase reductase (MTRR) A66G mutation in Yadav and Scheduled Caste (SC) population of Uttar Pradesh. MATERIALS AND METHODS: Blood samples were collected from 100 subjects after taking informed written consent and PCR-RFLP method was used for the analysis of A66G mutation. After NdeI digestion, 66-bp amplicon of normal allele was cut into 22- and 44-bp long fragments, whereas mutant G allele was not digested. RESULTS: Frequencies of genotypes in Yadav population AA, AG, and GG were 12%, 60%, and 28%, respectively, and in SC population, genotypic frequencies were 12% (AA), 52% (AG), and 36% (GG). CONCLUSION: MTRR gene A66G mutation is found to be polymorphic in both the target populations with G allele frequencies being 0.58 for Yadav and 0.62 for Scheduled Caste.
OBJECTIVE: This study was aimed to evaluate the Methionine synthase reductase (MTRR) A66G mutation in Yadav and Scheduled Caste (SC) population of Uttar Pradesh. MATERIALS AND METHODS: Blood samples were collected from 100 subjects after taking informed written consent and PCR-RFLP method was used for the analysis of A66G mutation. After NdeI digestion, 66-bp amplicon of normal allele was cut into 22- and 44-bp long fragments, whereas mutant G allele was not digested. RESULTS: Frequencies of genotypes in Yadav population AA, AG, and GG were 12%, 60%, and 28%, respectively, and in SC population, genotypic frequencies were 12% (AA), 52% (AG), and 36% (GG). CONCLUSION: MTRR gene A66G mutation is found to be polymorphic in both the target populations with G allele frequencies being 0.58 for Yadav and 0.62 for Scheduled Caste.