Literature DB >> 2380688

Purification and characterization of D-2-haloacid dehalogenase from Pseudomonas putida strain AJ1/23.

J M Smith1, K Harrison, J Colby.   

Abstract

A D-2-haloacid dehalogenase was isolated and purified to homogeneity from Pseudomonas putida strain AJ1/23. The enzyme catalysed the stereospecific dehalogenation of the D-isomer of 2-chloropropionate. Using a new ion-chromatograph assay, the enzyme was found to catalyse the dehalogenation of short-chain 2-halocarboxylic acids. Maximum enzyme activity occurred at pH 9.5 and 50 degrees C and the enzyme was insensitive to most -SH reagents. The enzyme has an Mr of about 135,000 and appears to be composed of four subunits of identical Mr.

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Year:  1990        PMID： 2380688     DOI： 10.1099/00221287-136-5-881

Source DB:  PubMed          Journal:  J Gen Microbiol        ISSN： 0022-1287


  19 in total

1.  Bacterial DL-2-haloacid dehalogenase from Pseudomonas sp. strain 113: gene cloning and structural comparison with D- and L-2-haloacid dehalogenases.

Authors:  V Nardi-Dei; T Kurihara; C Park; N Esaki; K Soda
Journal:  J Bacteriol       Date:  1997-07       Impact factor: 3.490

2.  Overexpression and feasible purification of thermostable L-2-halo acid dehalogenase of Pseudomonas sp. YL.

Authors:  J Q Liu; T Kurihara; V Nardi-Dei; T Okamura; N Esaki; K Soda
Journal:  Biodegradation       Date:  1995-09       Impact factor: 3.909

3.  Purification and characterization of a dehalogenase from Pseudomonas stutzeri DEH130 isolated from the marine sponge Hymeniacidon perlevis.

Authors:  Jinyou Zhang; Xupeng Cao; Yanjuan Xin; Song Xue; Wei Zhang
Journal:  World J Microbiol Biotechnol       Date:  2013-03-31       Impact factor: 3.312

4.  Cloning and partial sequencing of an operon encoding two Pseudomonas putida haloalkanoate dehalogenases of opposite stereospecificity.

Authors:  P T Barth; L Bolton; J C Thomson
Journal:  J Bacteriol       Date:  1992-04       Impact factor: 3.490

5.  Investigation of two evolutionarily unrelated halocarboxylic acid dehalogenase gene families.

Authors:  K E Hill; J R Marchesi; A J Weightman
Journal:  J Bacteriol       Date:  1999-04       Impact factor: 3.490

6.  Identification of the dimerization domain of dehalogenase IVa of Burkholderia cepacia MBA4.

Authors:  J S Tsang; B C Pang
Journal:  Appl Environ Microbiol       Date:  2000-08       Impact factor: 4.792

7.  Cloning and expression of a haloacid dehalogenase from Pseudomonas sp. strain 19 S.

Authors:  S Kocabiyik; B Aslan; R Müller
Journal:  Biodegradation       Date:  1995-09       Impact factor: 3.909

Review 8.  Bacterial dehalogenases: biochemistry, genetics, and biotechnological applications.

Authors:  S Fetzner; F Lingens
Journal:  Microbiol Rev       Date:  1994-12

9.  Comparative studies of genes encoding thermostable L-2-halo acid dehalogenase from Pseudomonas sp. strain YL, other dehalogenases, and two related hypothetical proteins from Escherichia coli.

Authors:  V Nardi-Dei; T Kurihara; T Okamura; J Q Liu; H Koshikawa; H Ozaki; Y Terashima; N Esaki; K Soda
Journal:  Appl Environ Microbiol       Date:  1994-09       Impact factor: 4.792

10.  Degradation of 3-chloro-2-methylpropionic acid by Xanthobacter sp. CIMW 99.

Authors:  M R Smith; W J van den Tweel; J A de Bont
Journal:  Appl Microbiol Biotechnol       Date:  1991-11       Impact factor: 4.813
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