| Literature DB >> 23806759 |
Jing Li1, Qing-Yu Zhang, Zhi-Hong Gao, Fei Wang, Ke Duan, Zheng-Wen Ye, Qing-Hua Gao.
Abstract
Anthracnose caused by Colletotrichum spp. is one of the most destructive diseases of cultivated strawberry (Fragaria×ananassa Duchesne) worldwide. The correlation between NBS-LRR genes, the largest class of known resistance genes, and strawberry anthracnose resistance has been elusive. BLAST search in NCBI identified 94 FvNBSs in the diploid genome of strawberry Fragaria vesca, with 67 of the TIR-NBS-LRR type. At least 36 FvNBSs were expressed, with 25% being non-coding genes. Two F. vesca ecotypes, HLJ and YW, showed great variations in both morphological and physiological responses upon C. gloeosporioides infection. qRT-PCR revealed that 5 of the 12 leaf-expressed FvNBSs displaying opposite transcription responses to C. gloeosporioides infection in two ecotypes. These results showed that the transcriptional responses of several FvNBSs were involved in the ecotype-specific responses to C. gloeosporioides in F. vesca. These FvNBSs hold potential in characterizing molecular components and developing novel markers associated with anthracnose resistance in strawberry.Entities:
Keywords: Colletotrichum gloeosporioides; Fragaria vesca; Genetic variation; LG; NBS–LRR; R genes; RGA; Resistance gene; Resistance genes; TIR Toll and mammalian Interleukin-1 receptor-like; TNL TIR–NBS–LRR; days post inoculation; dpi; hours post inoculation; hpi; linkage group; nucleotide-binding site and leucine-rich repeat; qPCR; qRT-PCR; quantitative reverse-transcription PCR; resistance gene analogs
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Year: 2013 PMID： 23806759 DOI： 10.1016/j.gene.2013.06.008
Source DB: PubMed Journal: Gene ISSN： 0378-1119 Impact factor: 3.688