| Literature DB >> 23804640 |
Hanh T Pham1, Françoise-Xavière Jousset, Jonathan Perreault, Hiroko Shike, Jozsef Szelei, Max Bergoin, Peter Tijssen.
Abstract
The transcription map of the Aedes albopictus densovirus (AalDNV) brevidensovirus was identified by Northern blotting, rapid amplification of cDNA ends (RACE) analysis, and RNase protection assays. AalDNV produced mRNAs of 3,359 (NS1), 3,345 (NS2), and 1,246 (VP) nucleotides. The two overlapping P7/7.4 NS promoters employed closely located alternate transcription initiation sites, positioned at either side of the NS1 initiation codon. All NS mRNAs coterminated with VP mRNA. All promoters, explored using luciferase assays, were functional in insect and human cell lines.Entities:
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Year: 2013 PMID: 23804640 PMCID: PMC3754130 DOI: 10.1128/JVI.01259-13
Source DB: PubMed Journal: J Virol ISSN: 0022-538X Impact factor: 5.103