BACKGROUND: microRNAs (miRNAs) that regulate proliferation, invasion and metastasis are considered to be the principal molecular basis of tumor heterogeneity. Breast cancer is not a homogeneous tissue. Thus, it is very important to perform microarray-based miRNA screening of tumors at different sites. METHODS: Breast tissue samples from the centers and edges of tumors of 30 patients were classified into 5 clinicopathological subtypes. In each group, 6 specimens were examined by microRNA array. All differential miRNAs were analyzed between the edges and centers of the tumors. RESULTS: Seventeen kinds of miRNAs were heterogeneously distributed in the tumors from different clinicopathological subtypes that included 1 kind of miRNA in Luminal A and Luminal B Her2+ subtypes, 4 kinds in Luminal A and Her2 overexpression subtypes, 6 kinds in Luminal B Ki67+ and Luminal B Her2+ subtypes, 2 kinds between Luminal B Ki67+ and triple-negative breast cancer (TNBC) subtypes, 2 kinds between Luminal B Her2+ and TNBC subtypes, and 2 kinds between Luminal B Ki67+, Luminal B Her2+, and TNBC subtypes. Twenty kinds of miRNAs were homogenously distributed in the tumors from different clinicopathological subtypes that included 6 kinds of miRNAs in Luminal B Ki67+ and Luminal B Her2+ subtypes, 1 kind in Luminal B Ki67+ and Her2 overexpression subtypes, 10 kinds between Luminal B Ki67+ and TNBC subtypes, 2 kinds in Luminal B Her2+ and TNBC subtypes, and 1 kind between Luminal B Ki67+, Luminal B Her2+, and TNBC subtypes. CONCLUSIONS: A total of 37 miRNAs were significantly distributed in tumors from the centers to edges, and in all clinicopathological subtypes.
BACKGROUND: microRNAs (miRNAs) that regulate proliferation, invasion and metastasis are considered to be the principal molecular basis of tumor heterogeneity. Breast cancer is not a homogeneous tissue. Thus, it is very important to perform microarray-based miRNA screening of tumors at different sites. METHODS: Breast tissue samples from the centers and edges of tumors of 30 patients were classified into 5 clinicopathological subtypes. In each group, 6 specimens were examined by microRNA array. All differential miRNAs were analyzed between the edges and centers of the tumors. RESULTS: Seventeen kinds of miRNAs were heterogeneously distributed in the tumors from different clinicopathological subtypes that included 1 kind of miRNA in Luminal A and Luminal B Her2+ subtypes, 4 kinds in Luminal A and Her2 overexpression subtypes, 6 kinds in Luminal B Ki67+ and Luminal B Her2+ subtypes, 2 kinds between Luminal B Ki67+ and triple-negative breast cancer (TNBC) subtypes, 2 kinds between Luminal B Her2+ and TNBC subtypes, and 2 kinds between Luminal B Ki67+, Luminal B Her2+, and TNBC subtypes. Twenty kinds of miRNAs were homogenously distributed in the tumors from different clinicopathological subtypes that included 6 kinds of miRNAs in Luminal B Ki67+ and Luminal B Her2+ subtypes, 1 kind in Luminal B Ki67+ and Her2 overexpression subtypes, 10 kinds between Luminal B Ki67+ and TNBC subtypes, 2 kinds in Luminal B Her2+ and TNBC subtypes, and 1 kind between Luminal B Ki67+, Luminal B Her2+, and TNBC subtypes. CONCLUSIONS: A total of 37 miRNAs were significantly distributed in tumors from the centers to edges, and in all clinicopathological subtypes.
Authors: Bruna M Sugita; Silma R Pereira; Rodrigo C de Almeida; Mandeep Gill; Akanksha Mahajan; Anju Duttargi; Saurabh Kirolikar; Paolo Fadda; Rubens S de Lima; Cicero A Urban; Kepher Makambi; Subha Madhavan; Simina M Boca; Yuriy Gusev; Iglenir J Cavalli; Enilze M S F Ribeiro; Luciane R Cavalli Journal: Oncotarget Date: 2019-10-22
Authors: Paolo Uva; Paolo Cossu-Rocca; Federica Loi; Giovanna Pira; Luciano Murgia; Sandra Orrù; Matteo Floris; Maria Rosaria Muroni; Francesca Sanges; Ciriaco Carru; Andrea Angius; Maria Rosaria De Miglio Journal: Int J Med Sci Date: 2018-03-09 Impact factor: 3.738
Authors: Patricia M M Ozawa; Evelyn Vieira; Débora S Lemos; Ingrid L Melo Souza; Silvio M Zanata; Vânia C Pankievicz; Thalita R Tuleski; Emanuel M Souza; Pryscilla F Wowk; Cícero de Andrade Urban; Flavia Kuroda; Rubens S Lima; Rodrigo C Almeida; Daniela F Gradia; Iglenir J Cavalli; Luciane R Cavalli; Danielle Malheiros; Enilze M S F Ribeiro Journal: Biomolecules Date: 2020-01-16