BACKGROUND: CORNEAS NEEDED FOR KERATOPLASTY CAN BE HARVESTED USING TWO TECHNIQUES: whole globe enucleation and in situ excision of the corneoscleral disc. This study evaluates the rate of microbial contamination of the donor cornea organ culture medium according to the method of retrieval. METHODS: All donor corneas of our cornea bank received between January 1, 2001 and December 31, 2009 put into organ culture and microbio-logically tested were prospectively analyzed for microbial contamination of the organ culture medium. RESULTS: 2,805 donor corneas could be included in this study in total. 975 of them were retrieved by whole globe enucleation (group 1) and 1,830 by in situ corneoscleral disc excision (group 2). 15 corneas of group 1 (1.5%) and 46 corneas of group 2 (2.5%) showed a contamination of the organ culture medium. The difference was shown not to be statistically significant (p = 0.082). CONCLUSION: The rate of microbial contamination in organ-cultured donor corneas does not seem to be dependent on the method of their retrieval.
BACKGROUND: CORNEAS NEEDED FOR KERATOPLASTY CAN BE HARVESTED USING TWO TECHNIQUES: whole globe enucleation and in situ excision of the corneoscleral disc. This study evaluates the rate of microbial contamination of the donor cornea organ culture medium according to the method of retrieval. METHODS: All donor corneas of our cornea bank received between January 1, 2001 and December 31, 2009 put into organ culture and microbio-logically tested were prospectively analyzed for microbial contamination of the organ culture medium. RESULTS: 2,805 donor corneas could be included in this study in total. 975 of them were retrieved by whole globe enucleation (group 1) and 1,830 by in situ corneoscleral disc excision (group 2). 15 corneas of group 1 (1.5%) and 46 corneas of group 2 (2.5%) showed a contamination of the organ culture medium. The difference was shown not to be statistically significant (p = 0.082). CONCLUSION: The rate of microbial contamination in organ-cultured donor corneas does not seem to be dependent on the method of their retrieval.
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Keywords:
Cornea donation; In situ corneoscleral disc excision; Microbiology; Organ culture; Whole globe enucleation
Authors: Stephan J Linke; Otto H Fricke; Mau-Thek Eddy; Jürgen Bednarz; Vasyl Druchkiv; Paul-Michael Kaulfers; Birgit Wulff; Klaus Püschel; Gisbert Richard; Olaf J C Hellwinkel Journal: Cornea Date: 2013-02 Impact factor: 2.651
Authors: Tobias Röck; Johanna Landenberger; Michael Buhl; Efdal Yoeruek; Karl Ulrich Bartz-Schmidt; Matthias Bramkamp; Gunnar Blumenstock; Daniel Röck Journal: Medicine (Baltimore) Date: 2018-09 Impact factor: 1.817