Literature DB >> 23801118

Identification and characterization of P GCW14 : a novel, strong constitutive promoter of Pichia pastoris.

Shuli Liang1, Chengjuan Zou, Ying Lin, Xuanwei Zhang, Yanrui Ye.   

Abstract

The available promoters in the Pichia pastoris expression platform are still limited. We selected and identified a novel strong constitutive promoter, P GCW14 , and tested its promoter activity using enhanced green fluorescent protein (EGFP) as a reporter. Potential promoter regions of P GCW14 were cloned upstream of the EGFP gene and promoter activity was analyzed by measuring fluorescence intensity. P GCW14 exhibited significantly stronger promoter activity than the classic strong constitutive promoters P TEF1 and P GAP under various carbon sources, suggesting that P GCW14 is a strong and constitutive promoter. Hence, P GCW14 can be used as a promoter for high-level expression of heterologous proteins.

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Year:  2013        PMID： 23801118     DOI： 10.1007/s10529-013-1265-8

Source DB:  PubMed          Journal:  Biotechnol Lett        ISSN： 0141-5492            Impact factor:   2.461


  16 in total

1.  Optimization of Cephalosporin C Acylase Expression in Escherichia coli by High-Throughput Screening a Constitutive Promoter Mutant library.

Authors:  Hongxu Sun; Tianjiao Liu; Hui Luo; Zihao Nie; Yanhong Chang; Huimin Yu; Zhongyao Shen
Journal:  Appl Biochem Biotechnol       Date:  2021-01-06       Impact factor: 2.926

2.  Identification of a novel promoter for driving antibiotic-resistant genes to reduce the metabolic burden during protein expression and effectively select multiple integrations in Pichia Pastoris.

Authors:  Qi Shen; Zhuang Yu; Xiao-Ting Zhou; Shi-Jia Zhang; Shu-Ping Zou; Neng Xiong; Ya-Ping Xue; Zhi-Qiang Liu; Yu-Guo Zheng
Journal:  Appl Microbiol Biotechnol       Date:  2021-04-05       Impact factor: 4.813

3.  Combined use of GAP and AOX1 promoters and optimization of culture conditions to enhance expression of Rhizomucor miehei lipase.

Authors:  Dong He; Wen Luo; Zhiyuan Wang; Pengmei Lv; Zhenhong Yuan
Journal:  J Ind Microbiol Biotechnol       Date:  2015-05-27       Impact factor: 3.346

Review 4.  Expanding the promoter toolbox for metabolic engineering of methylotrophic yeasts.

Authors:  Chunxiao Yan; Wei Yu; Lun Yao; Xiaoyu Guo; Yongjin J Zhou; Jiaoqi Gao
Journal:  Appl Microbiol Biotechnol       Date:  2022-05-11       Impact factor: 4.813

5.  Engineering of Promoters for Gene Expression in Pichia pastoris.

Authors:  Thomas Vogl
Journal:  Methods Mol Biol       Date:  2022

6.  Isolation and evaluation of strong endogenous promoters for the heterologous expression of proteins in Pichia pastoris.

Authors:  Yafei Zhang; Shengyan Wang; Lingfang Lu; Chenshan Zhang; Fan Cai; Yao Lin; Yide Huang
Journal:  World J Microbiol Biotechnol       Date:  2022-09-19       Impact factor: 4.253

7.  Comparative genomics and transcriptomics of Pichia pastoris.

Authors:  Kerry R Love; Kartik A Shah; Charles A Whittaker; Jie Wu; M Catherine Bartlett; Duanduan Ma; Rachel L Leeson; Margaret Priest; Jonathan Borowsky; Sarah K Young; J Christopher Love
Journal:  BMC Genomics       Date:  2016-08-05       Impact factor: 3.969

Review 8.  Protein expression in Pichia pastoris: recent achievements and perspectives for heterologous protein production.

Authors:  Mudassar Ahmad; Melanie Hirz; Harald Pichler; Helmut Schwab
Journal:  Appl Microbiol Biotechnol       Date:  2014-04-18       Impact factor: 4.813

9.  Mit1 Transcription Factor Mediates Methanol Signaling and Regulates the Alcohol Oxidase 1 (AOX1) Promoter in Pichia pastoris.

Authors:  Xiaolong Wang; Qi Wang; Jinjia Wang; Peng Bai; Lei Shi; Wei Shen; Mian Zhou; Xiangshan Zhou; Yuanxing Zhang; Menghao Cai
Journal:  J Biol Chem       Date:  2016-01-31       Impact factor: 5.157

10.  Characterization of a panARS-based episomal vector in the methylotrophic yeast Pichia pastoris for recombinant protein production and synthetic biology applications.

Authors:  Andrea Camattari; Amelia Goh; Lian Yee Yip; Andy Hee Meng Tan; Sze Wai Ng; Anthony Tran; Gaowen Liu; Ivan Liachko; Maitreya J Dunham; Giulia Rancati
Journal:  Microb Cell Fact       Date:  2016-08-11       Impact factor: 5.328
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