BACKGROUND: Measurement of immature platelets was introduced into routine diagnostics by Sysmex as immature platelet fraction (IPF) some years ago and recently by Abbott as reticulated platelet fraction (rPT). Here, we compare both methods. METHODS: We evaluated the precision and agreement of these parameters between Sysmex XE-5000 and Abbott CD-Sapphire in three distinct thrombocytopaenic cohorts: 30 patients with beginning thrombocytopaenia and 64 patients with recovering platelets (PLT) after chemotherapy, 16 patients with immune thrombocytopaenia (ITP) or heparin-induced thrombocytopaenia type 2 (HIT) and 110 additional normal controls. Furthermore, we analysed, how IPF/rPT differed between these thrombocytopaenic cohorts and controls. RESULTS: Both analysers demonstrated acceptable overall precision (repeatability) of IPF/rPT with lower precision at low PLT counts. IPF/rPT artificially increased during storage of blood samples overnight. Inter-instrument comparison showed a moderate correlation (Pearson r²=0.38) and a systematic bias of 1.04 towards higher IPF-values with the XE-5000. IPF/rPT was highest in recovering thrombopoesis after chemotherapy and moderately increased in ITP/HIT. The normal range deduced from control samples was much narrower with CD-Sapphire (1.0%-3.8%, established here for the first time) in comparison to XE-5000 (0.8%-7.9%) leading to a smaller overlap of samples with increased PLT turnover and normal controls. CONCLUSIONS: IPF and rPT both give useful information on PLT turnover, although the two analysers only show a moderate inter-instrument correlation and have different reference ranges. A better separation of patient groups with high PLT turnover like ITP/HIT from normal controls is obtained by CD-Sapphire.
BACKGROUND: Measurement of immature platelets was introduced into routine diagnostics by Sysmex as immature platelet fraction (IPF) some years ago and recently by Abbott as reticulated platelet fraction (rPT). Here, we compare both methods. METHODS: We evaluated the precision and agreement of these parameters between Sysmex XE-5000 and Abbott CD-Sapphire in three distinct thrombocytopaenic cohorts: 30 patients with beginning thrombocytopaenia and 64 patients with recovering platelets (PLT) after chemotherapy, 16 patients with immune thrombocytopaenia (ITP) or heparin-induced thrombocytopaenia type 2 (HIT) and 110 additional normal controls. Furthermore, we analysed, how IPF/rPT differed between these thrombocytopaenic cohorts and controls. RESULTS: Both analysers demonstrated acceptable overall precision (repeatability) of IPF/rPT with lower precision at low PLT counts. IPF/rPT artificially increased during storage of blood samples overnight. Inter-instrument comparison showed a moderate correlation (Pearson r²=0.38) and a systematic bias of 1.04 towards higher IPF-values with the XE-5000. IPF/rPT was highest in recovering thrombopoesis after chemotherapy and moderately increased in ITP/HIT. The normal range deduced from control samples was much narrower with CD-Sapphire (1.0%-3.8%, established here for the first time) in comparison to XE-5000 (0.8%-7.9%) leading to a smaller overlap of samples with increased PLT turnover and normal controls. CONCLUSIONS: IPF and rPT both give useful information on PLT turnover, although the two analysers only show a moderate inter-instrument correlation and have different reference ranges. A better separation of patient groups with high PLT turnover like ITP/HIT from normal controls is obtained by CD-Sapphire.
Authors: Rodolfo Monteiro Enz Hubert; Melina Veiga Rodrigues; Bruna Dolci Andreguetto; Thiago M Santos; Maria de Fátima Pereira Gilberti; Vagner de Castro; Joyce M Annichino-Bizzacchi; Desanka Dragosavac; Marco Antonio Carvalho-Filho; Erich Vinicius De Paula Journal: Sci Rep Date: 2015-01-26 Impact factor: 4.379
Authors: Noreen van der Linden; Lieke J J Klinkenberg; Steven J R Meex; Erik A M Beckers; Norbert C J de Wit; Lenneke Prinzen Journal: Eur J Haematol Date: 2014-04-25 Impact factor: 2.997
Authors: Catherine Angénieux; Blandine Maître; Anita Eckly; François Lanza; Christian Gachet; Henri de la Salle Journal: PLoS One Date: 2016-01-25 Impact factor: 3.240
Authors: Stefan J Schaller; Kristina Fuest; Bernhard Ulm; Sebastian Schmid; Catherina Bubb; Rüdiger von Eisenhart-Rothe; Helmut Friess; Chlodwig Kirchhoff; Thomas Stadlbauer; Peter Luppa; Manfred Blobner; Bettina Jungwirth Journal: Trials Date: 2020-08-18 Impact factor: 2.279