Literature DB >> 2379837

CelS: a novel endoglucanase identified from Erwinia carotovora subsp. carotovora.

H T Saarilahti1, B Henrissat, E T Palva.   

Abstract

A plasmid clone expressing a beta(1,4)-glucan glucanohydrolase (EC 3.2.1.4; endoglucanase) in Escherichia coli was isolated from a genomic library of Erwinia carotovora subsp. carotovora. The DNA segment carrying the corresponding structural gene, named celS, contained an open reading frame encoding a 264-amino acid (aa) polypeptide. The N-terminal aa sequence of CelS showed that the protein was synthesized with a 32-aa cleavable signal peptide. The mature 232-aa CelS had a calculated Mr of 26,228 and pI of 5.5. The pH optimum was about 6.8 and the temperature optimum was between 45 and 55 degrees C. Comparison of the aa sequence of CelS by hydrophobic cluster analysis with a range of cellulases and other quasi-isofunctional enzymes revealed only very limited sequence similarities, suggesting that the CelS protein may represent the first member of an additional cellulase family.

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Year:  1990        PMID： 2379837     DOI： 10.1016/0378-1119(90)90433-r

Source DB:  PubMed          Journal:  Gene        ISSN： 0378-1119            Impact factor:   3.688


  12 in total

Review 1.  Domains in microbial beta-1, 4-glycanases: sequence conservation, function, and enzyme families.

Authors:  N R Gilkes; B Henrissat; D G Kilburn; R C Miller; R A Warren
Journal:  Microbiol Rev       Date:  1991-06

2.  An endoglucanase, EglA, from the hyperthermophilic archaeon Pyrococcus furiosus hydrolyzes beta-1,4 bonds in mixed-linkage (1-->3),(1-->4)-beta-D-glucans and cellulose.

Authors:  M W Bauer; L E Driskill; W Callen; M A Snead; E J Mathur; R M Kelly
Journal:  J Bacteriol       Date:  1999-01       Impact factor: 3.490

3.  Molecular characterization and heterologous expression of the gene encoding a low-molecular-mass endoglucanase from Trichoderma reesei QM9414.

Authors:  H Okada; K Tada; T Sekiya; K Yokoyama; A Takahashi; H Tohda; H Kumagai; Y Morikawa
Journal:  Appl Environ Microbiol       Date:  1998-02       Impact factor: 4.792

4.  Two genes encoding an endoglucanase and a cellulose-binding protein are clustered and co-regulated by a TTA codon in Streptomyces halstedii JM8.

Authors:  A L Garda; J M Fernández-Abalos; P Sánchez; A Ruiz-Arribas; R I Santamaría
Journal:  Biochem J       Date:  1997-06-01       Impact factor: 3.857

5.  Structure and regulation of the Erwinia carotovora subspecies carotovora SCC3193 cellulase gene celV1 and the role of cellulase in phytopathogenicity.

Authors:  A Mäe; R Heikinheimo; E T Palva
Journal:  Mol Gen Genet       Date:  1995-04-10

6.  Purification, characterization, and molecular analysis of thermostable cellulases CelA and CelB from Thermotoga neapolitana.

Authors:  J D Bok; D A Yernool; D E Eveleigh
Journal:  Appl Environ Microbiol       Date:  1998-12       Impact factor: 4.792

7.  Identification of Glu-120 as the catalytic nucleophile in Streptomyces lividans endoglucanase celB.

Authors:  D L Zechel; S He; C Dupont; S G Withers
Journal:  Biochem J       Date:  1998-11-15       Impact factor: 3.857

8.  Expression of pehA-bla gene fusions in Erwinia carotovora subsp. carotovora and isolation of regulatory mutants affecting polygalacturonase production.

Authors:  H T Saarilahti; M Pirhonen; M B Karlsson; D Flego; E T Palva
Journal:  Mol Gen Genet       Date:  1992-07

9.  Molecular analysis of the major cellulase (CelV) of Erwinia carotovora: evidence for an evolutionary "mix-and-match" of enzyme domains.

Authors:  V J Cooper; G P Salmond
Journal:  Mol Gen Genet       Date:  1993-11

10.  Cloning, Expression, and Characterization of a Thermophilic Endoglucanase, AcCel12B from Acidothermus cellulolyticus 11B.

Authors:  Junling Wang; Gui Gao; Yuwei Li; Liangzhen Yang; Yanli Liang; Hanyong Jin; Weiwei Han; Yan Feng; Zuoming Zhang
Journal:  Int J Mol Sci       Date:  2015-10-22       Impact factor: 5.923
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