Literature DB >> 2379835

Proposal for sequencing DNA using ligation of hexamers to generate sequential elongation primers (SPEL-6).

W Szybalski1.   

Abstract

The efficiency of the directed-priming proposal of Studier [Proc. Natl. Acad. Sci. USA 86 (1989) 6917-1921] can be improved upon by employing a library of less than 4094 hexamers, allowing for the easy construction of all required primers using template-directed automated ligation. This 'top-down' sequencing procedure should be several times more efficient than the present 'bottom-up' procedures [see Watson, Science 248 (1990) 44-49].

Mesh:

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Year:  1990        PMID: 2379835     DOI: 10.1016/0378-1119(90)90458-4

Source DB:  PubMed          Journal:  Gene        ISSN: 0378-1119            Impact factor:   3.688


  7 in total

1.  DNA sequencing using differential extension with nucleotide subsets (DENS).

Authors:  M C Raja; D Zevin-Sonkin; J Shwartzburd; T A Rozovskaya; I A Sobolev; O Chertkov; V Ramanathan; L Lvovsky; L E Ulanovsky
Journal:  Nucleic Acids Res       Date:  1997-02-15       Impact factor: 16.971

2.  A DNA sequencing strategy that requires only five bases of known terminal sequence for priming.

Authors:  D J Fu; N E Broude; H Köster; C L Smith; C R Cantor
Journal:  Proc Natl Acad Sci U S A       Date:  1995-10-24       Impact factor: 11.205

3.  DNA sequencing: modular primers assembled from a library of hexamers or pentamers.

Authors:  L E Kotler; D Zevin-Sonkin; I A Sobolev; A D Beskin; L E Ulanovsky
Journal:  Proc Natl Acad Sci U S A       Date:  1993-05-01       Impact factor: 11.205

4.  On the mechanism of the modular primer effect.

Authors:  A D Beskin; D Zevin-Sonkin; I A Sobolev; L E Ulanovsky
Journal:  Nucleic Acids Res       Date:  1995-08-11       Impact factor: 16.971

5.  Fluorescence-based DNA sequencing with hexamer primers.

Authors:  W Hou; L M Smith
Journal:  Nucleic Acids Res       Date:  1993-07-11       Impact factor: 16.971

6.  Dynamic assembly of primers on nucleic acid templates.

Authors:  Nicole A Leal; Makoto Sukeda; Steven A Benner
Journal:  Nucleic Acids Res       Date:  2006-09-08       Impact factor: 16.971

7.  DNA ligase-mediated translation of DNA into densely functionalized nucleic acid polymers.

Authors:  Ryan Hili; Jia Niu; David R Liu
Journal:  J Am Chem Soc       Date:  2012-12-26       Impact factor: 15.419

  7 in total

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