| Literature DB >> 23791757 |
Young-min Ha1, Su-Hyun Chun, Seung-taek Hong, Yun-chang Koo, Hee-don Choi, Kwang-won Lee.
Abstract
We studied the immune-modulating effect of Maillard-type lysozyme-galactomannan conjugate (LGC). LGC significantly induced nitric oxide, and expressions of tumor necrosis factor (TNF)-α, interleukin (IL)-1β and IL-8 on the murine macrophage Raw 264.7 cell line. In the mechanism of LGC, while extracellular signal-regulated kinase (ERK) was important for the induction of TNF-α, IL-1β and IL-8, the phosphorylation of C-Jun NH2-termianl kinase (JNK) contributed to the induction of TNF-α and IL-1β to a greater degree. These cytokines were less sensitive to the inhibition of p38. Nuclear factor (NF)-κB was involved in the induction of TNF-α and IL-1β. These data indicate that LGC has immune-modulating effects via JNK, ERK and NF-κB pathways, and that LGC may contribute to host immune defense.Entities:
Keywords: 3-(4,5-dimethylthazol-2-yl)-2,5-diphenyl tetrazolium bromide; C-Jun NH2-termianl kinase; DMEM; Dulbecco's modified Eagle's medium; ECL; ERK; FBS; G; Galactomannan; IFN-γ; IL; Immune-modulating effect; JNK; L; LGC; Lysozyme; MAPKs; MTT; Maillard reaction; NF-κB; NO; PVDF; RH; TGF-β; TNF-α; electrochemiluminescent; extracellular signal-regulated kinase; fetal bovine serum; galactomannan; iNOS; inducible nitric oxide synthase; interferon-γ; interleukin; lysozyme; lysozyme-galactomannan conjugate; mitogen-activated protein kinases; nitric oxide; nuclear factor-κB; polyvinylidene difluoride; relative humidity; transforming growth factor-β; tumor necrosis factor
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Year: 2013 PMID： 23791757 DOI： 10.1016/j.ijbiomac.2013.06.007
Source DB: PubMed Journal: Int J Biol Macromol ISSN： 0141-8130 Impact factor: 6.953