| Literature DB >> 23791093 |
Nataliya M Mishina1, Kseniya N Markvicheva, Dmitry S Bilan, Mikhail E Matlashov, Marina V Shirmanova, David Liebl, Carsten Schultz, Sergey Lukyanov, Vsevolod V Belousov.
Abstract
The fluorescent sensor HyPer allows monitoring of intracellular H2O2 levels with a high degree of sensitivity and specificity. Here, we provide a detailed protocol of ratiometric imaging of H2O2 produced by cells during phagocytosis, including instructions for experiments on different commercial confocal systems, namely, Leica SP2, Leica SP5, and Carl Zeiss LSM, as well as wide-field Leica 6000 microscope. The general experimental scheme is easily adaptable for imaging H2O2 production by various cell types under a variety of conditions.Entities:
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Year: 2013 PMID： 23791093 DOI： 10.1016/B978-0-12-405883-5.00003-X
Source DB: PubMed Journal: Methods Enzymol ISSN： 0076-6879 Impact factor: 1.600