Wei Li1, Ying Zhao, Xinna Li. 1. Department of Pharmacy, Harbin Commercial University, Harbin 150076, China.
Abstract
OBJECTIVE: To explore the inhibitory effect of Zishenshengxue capsule (ZSC) on myelosuppression in mice induced by cyclophosphamide. METHODS: Kunming mice were randomly assigned into a control group, myelosuppression group, or groups for mice with myelosuppression receiving high dose ZSC, middle dose ZSC, low dose ZSC or Yixuesheng. Myelosuppression was induced by peritoneal injection of cyclophosphamide. ZSC and cyclophosphamide were administered simultaneously. The numbers of peripheral blood cells and bone marrow karyocytes were counted. Cell proliferation activity and colony formation of granulocyte-monocyte series hemopoietic progenitor cells (C-GMs) and cell cycle were detected. RESULTS: The numbers of white blood cells in the middle and high dose ZSC groups were significantly increased at the 12th and 13th day (P < 0.01) and 0.01) compared with the myelosuppression group. C-GMs of middle and high dose ZSC groups significantly increased (P < 0.01). The percentage of G1 phase in the high dose ZSC group decreased (P < 0.01) and the percentage of S and G2 phase increased (P < 0.01). CONCLUSION: ZSC increased the numbers of peripheral white blood cells, bone marrow karyocytes and C-GMs. ZSC also increased cell proliferation activity and removed the G1 phase block. Thus, ZSC could reduce myelosuppression in mice induced by cyclophosphamide.
OBJECTIVE: To explore the inhibitory effect of Zishenshengxue capsule (ZSC) on myelosuppression in mice induced by cyclophosphamide. METHODS: Kunming mice were randomly assigned into a control group, myelosuppression group, or groups for mice with myelosuppression receiving high dose ZSC, middle dose ZSC, low dose ZSC or Yixuesheng. Myelosuppression was induced by peritoneal injection of cyclophosphamide. ZSC and cyclophosphamide were administered simultaneously. The numbers of peripheral blood cells and bone marrow karyocytes were counted. Cell proliferation activity and colony formation of granulocyte-monocyte series hemopoietic progenitor cells (C-GMs) and cell cycle were detected. RESULTS: The numbers of white blood cells in the middle and high dose ZSC groups were significantly increased at the 12th and 13th day (P < 0.01) and 0.01) compared with the myelosuppression group. C-GMs of middle and high dose ZSC groups significantly increased (P < 0.01). The percentage of G1 phase in the high dose ZSC group decreased (P < 0.01) and the percentage of S and G2 phase increased (P < 0.01). CONCLUSION:ZSC increased the numbers of peripheral white blood cells, bone marrow karyocytes and C-GMs. ZSC also increased cell proliferation activity and removed the G1 phase block. Thus, ZSC could reduce myelosuppression in mice induced by cyclophosphamide.