INTRODUCTION: Nonsyndromic cleft lip and cleft palate (CL/P) is associated with environmental, nutritional, and genetic factors. Maternal polymorphisms in the methylenetetrahydrofolate reductase (MTHFR) gene have been associated with CL/P. OBJECTIVES: To determine the relationship between the risk of having a child with CL/P and maternal C677T and A1298C MTHFR polymorphisms, the intake of folate supplements, and exposure to environmental factors during the first trimester of pregnancy, a case-control study of Mexican mothers (88 case mothers and 116 control mothers) was conducted. METHODS: A questionnaire was used to assess exposure to environmental factors. The C677T and A1298C polymorphisms were identified by polymerase chain reaction with restriction fragment length polymorphism. RESULTS: Mothers with the 677CT or 677TT genotype had a higher risk of having a child with CL/P than mothers with the 677CC genotype (odds ratio [OR], 2.4; 95% confidence interval [CI], 1.1-5.7). An increased risk of having a child with CL/P was associated with the lack of folate supplementation during the first trimester of pregnancy (OR, 3.8; 95% CI, 1.9-7.6), and this risk was greater in the mothers with the 677TT or 677CT genotype than mothers who reported taking folate supplements and had the 677CC genotype (OR, 11.2; 95% CI, 3.3-37.5). Pesticide exposure was associated with CL/P. There was no significant association between either the A1298C variant or tobacco exposure and the risk of CL/P. CONCLUSION: These results suggest that gene-environment interactions play an important role in the development of CL/P.
INTRODUCTION:Nonsyndromic cleft lip and cleft palate (CL/P) is associated with environmental, nutritional, and genetic factors. Maternal polymorphisms in the methylenetetrahydrofolate reductase (MTHFR) gene have been associated with CL/P. OBJECTIVES: To determine the relationship between the risk of having a child with CL/P and maternal C677T and A1298CMTHFR polymorphisms, the intake of folate supplements, and exposure to environmental factors during the first trimester of pregnancy, a case-control study of Mexican mothers (88 case mothers and 116 control mothers) was conducted. METHODS: A questionnaire was used to assess exposure to environmental factors. The C677T and A1298C polymorphisms were identified by polymerase chain reaction with restriction fragment length polymorphism. RESULTS: Mothers with the 677CT or 677TT genotype had a higher risk of having a child with CL/P than mothers with the 677CC genotype (odds ratio [OR], 2.4; 95% confidence interval [CI], 1.1-5.7). An increased risk of having a child with CL/P was associated with the lack of folate supplementation during the first trimester of pregnancy (OR, 3.8; 95% CI, 1.9-7.6), and this risk was greater in the mothers with the 677TT or 677CT genotype than mothers who reported taking folate supplements and had the 677CC genotype (OR, 11.2; 95% CI, 3.3-37.5). Pesticide exposure was associated with CL/P. There was no significant association between either the A1298C variant or tobacco exposure and the risk of CL/P. CONCLUSION: These results suggest that gene-environment interactions play an important role in the development of CL/P.
Authors: Claudia Ojeda-Granados; Martina Barchitta; Maria Clara La Rosa; Claudia La Mastra; Sonia Roman; Arturo Panduro; Antonella Agodi; Andrea Maugeri Journal: Nutrients Date: 2022-04-12 Impact factor: 6.706
Authors: Queletzu Aspra; Brenda Cabrera-Mendoza; Mirna Edith Morales-Marín; Carla Márquez; Carlos Chicalote; Ana Ballesteros; Miriam Aguilar; Xochitl Castro; Amalia Gómez-Cotero; Ana María Balboa-Verduzco; Lilia Albores-Gallo; Omar Nafate-López; Carlos Alfonso Marcín-Salazar; Patricia Sánchez; Nuria Lanzagorta-Piñol; Fernando Omar López-Armenta; Humberto Nicolini Journal: Children (Basel) Date: 2022-03-25