Literature DB >> 2377599

Molecular cloning and characterization of human pyruvate dehydrogenase beta subunit gene.

K Koike1, Y Urata, M Koike.   

Abstract

A genomic clone encompassing the entire gene for the human pyruvate dehydrogenase beta subunit (PDH beta) has been isolated by screening a leukocyte genomic library with a nick-translated human foreskin fibroblast PDH beta cDNA probe. The 18-kilobase clone was characterized by restriction enzyme analysis, extensive DNA sequencing, and primer-extension analysis. The PDH beta structural gene is composed of 10 exons and 9 introns. All intron-exon splice junctions follow the GT/AG rule. The Alu family was found in introns 2 and 8. The 5' flanking region of the PDH beta gene contains a "CAAT" consensus promoter sequence but no "TATA" sequence. Primer-extension analysis indicated that the PDH beta gene transcription start site is an adenine residue located 132 bases upstream from the initiation codon in exon 1.

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Year:  1990        PMID： 2377599      PMCID： PMC54373          DOI： 10.1073/pnas.87.15.5594

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  13 in total

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Journal:  J Mol Biol       Date:  1975-11-05       Impact factor: 5.469

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Journal:  Biochem J       Date:  1988-08-15       Impact factor: 3.857

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Journal:  J Biol Chem       Date:  1969-07-10       Impact factor: 5.157

Review 5.  Structure, assembly and function of mammalian alpha-keto acid dehydrogenase complexes.

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Authors:  R Breathnach; P Chambon
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Authors:  M Hamada; T Hiraoka; K Koike; K Ogasahara; T Kanzaki
Journal:  J Biochem       Date:  1976-06       Impact factor: 3.387

8.  DNA sequencing with chain-terminating inhibitors.

Authors:  F Sanger; S Nicklen; A R Coulson
Journal:  Proc Natl Acad Sci U S A       Date:  1977-12       Impact factor: 11.205

9.  Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.

Authors:  J M Chirgwin; A E Przybyla; R J MacDonald; W J Rutter
Journal:  Biochemistry       Date:  1979-11-27       Impact factor: 3.162

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Authors:  C F Hawkins; A Borges; R N Perham
Journal:  FEBS Lett       Date:  1989-09-11       Impact factor: 4.124
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  5 in total

1.  New nucleotide sequence data on the EMBL File Server.

Authors: 
Journal:  Nucleic Acids Res       Date:  1990-10-25       Impact factor: 16.971

2.  A novel, rapid in cell RNA amplification technique for the detection of low copy mRNA transcripts.

Authors:  V Uhlmann; A Rolfs; E Mix; I Silva; J Hully; L Lu; K Lohman; D Howells; S Picton; J J O'Leary
Journal:  Mol Pathol       Date:  1998-06

3.  Characterization of the transcriptional regulatory region of the human dihydrolipoamide dehydrogenase gene.

Authors:  G L Johanning; J I Morris; K T Madhusudhan; D Samols; M S Patel
Journal:  Proc Natl Acad Sci U S A       Date:  1992-11-15       Impact factor: 11.205

4.  Lactate-mediated mitoribosomal defects impair mitochondrial oxidative phosphorylation and promote hepatoma cell invasiveness.

Authors:  Young-Kyoung Lee; Jin J Lim; Un-Woo Jeoun; Seongki Min; Eun-Beom Lee; So Mee Kwon; Changhan Lee; Gyesoon Yoon
Journal:  J Biol Chem       Date:  2017-10-04       Impact factor: 5.157

5.  Molecular Characterization and Transcriptional Regulation Analysis of the Bovine PDHB Gene.

Authors:  Anning Li; Yaran Zhang; Zhidong Zhao; Mingming Wang; Linsen Zan
Journal:  PLoS One       Date:  2016-07-05       Impact factor: 3.240
  5 in total

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