Literature DB >> 23775739

Chimerism testing by quantitative PCR using Indel markers.

Ketevan Gendzekhadze1, Laima Gaidulis, David Senitzer.   

Abstract

Engraftment monitoring is critical for patients after Hematopoietic Stem Cell Transplantation (HSCT). Complete donor chimerism is the goal; therefore, early detection of rejection and relapse is crucial for guiding the patient post HSCT treatment. Quantitative PCR for chimerism testing has been reported to be highly sensitive. In this chapter we discuss the quantitative PCR (qPCR) method using 34 Indel (Insertion and Deletion) genetic markers spread over 20 different chromosomes.

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Year:  2013        PMID: 23775739     DOI: 10.1007/978-1-62703-493-7_11

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN: 1064-3745


  1 in total

1.  Assessment of Minimal Residual Disease by Next Generation Sequencing in Peripheral Blood as a Complementary Tool for Personalized Transplant Monitoring in Myeloid Neoplasms.

Authors:  Paula Aguirre-Ruiz; Beñat Ariceta; María Cruz Viguria; María Teresa Zudaire; Zuriñe Blasco-Iturri; Patricia Arnedo; Almudena Aguilera-Diaz; Axier Jauregui; Amagoia Mañú; Felipe Prosper; María Carmen Mateos; Marta Fernández-Mercado; María José Larráyoz; Margarita Redondo; María José Calasanz; Iria Vázquez; Eva Bandrés
Journal:  J Clin Med       Date:  2020-11-25       Impact factor: 4.241

  1 in total

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