OBJECTIVES: Composites are the principal material for tooth cavity restorations due to their esthetics and direct-filling capabilities. However, composites accumulate biofilms in vivo, and secondary caries due to biofilm acids is the main cause of restoration failure. The objectives of this study were to: (1) synthesize new antibacterial monomers and (2) develop nanocomposite containing nanoparticles of amorphous calcium phosphate (NACP) and antibacterial monomer. METHODS: Two new antibacterial monomers were synthesized: dimethylaminohexane methacrylate (DMAHM) with a carbon chain length of 6, and dimethylaminododecyl methacrylate (DMADDM) with a chain length of 12. A spray-drying technique was used to make NACP. DMADDM was incorporated into NACP nanocomposite at mass fractions of 0%, 0.75%, 1.5%, 2.25% and 3%. A flexural test was used to measure composite strength and elastic modulus. A dental plaque microcosm biofilm model with human saliva as inoculum was used to measure viability, metabolic activity, and lactic acid production of biofilms on composites. RESULTS: The new DMAHM was more potent than a previous quaternary ammonium dimethacrylate (QADM). DMADDM was much more strongly antibacterial than DMAHM. The new DMADDM-NACP nanocomposite had strength similar to that of composite control (p>0.1). At 3% DMADDM in the composite, the metabolic activity of adherent biofilms was reduced to 5% of that on composite control. Lactic acid production by biofilms on composite containing 3% DMADDM was reduced to only 1% of that on composite control. Biofilm colony-forming unit (CFU) counts on composite with 3% DMADDM were reduced by 2-3 orders of magnitude. SIGNIFICANCE: New antibacterial monomers were synthesized, and the carbon chain length had a strong effect on antibacterial efficacy. The new DMADDM-NACP nanocomposite possessed potent anti-biofilm activity without compromising load-bearing properties, and is promising for antibacterial and remineralizing dental restorations to inhibit secondary caries.
OBJECTIVES: Composites are the principal material for tooth cavity restorations due to their esthetics and direct-filling capabilities. However, composites accumulate biofilms in vivo, and secondary caries due to biofilm acids is the main cause ofrestoration failure. The objectives of this study were to: (1) synthesize new antibacterial monomers and (2) develop nanocomposite containing nanoparticles of amorphous calcium phosphate (NACP) and antibacterial monomer. METHODS: Two new antibacterial monomers were synthesized: dimethylaminohexane methacrylate (DMAHM) with a carbon chain length of 6, and dimethylaminododecyl methacrylate (DMADDM) with a chain length of 12. A spray-drying technique was used to make NACP. DMADDM was incorporated into NACP nanocomposite at mass fractions of 0%, 0.75%, 1.5%, 2.25% and 3%. A flexural test was used to measure composite strength and elastic modulus. A dental plaque microcosm biofilm model with human saliva as inoculum was used to measure viability, metabolic activity, and lactic acid production of biofilms on composites. RESULTS: The new DMAHM was more potent than a previous quaternary ammonium dimethacrylate (QADM). DMADDM was much more strongly antibacterial than DMAHM. The new DMADDM-NACP nanocomposite had strength similar to that of composite control (p>0.1). At 3% DMADDM in the composite, the metabolic activity of adherent biofilms was reduced to 5% of that on composite control. Lactic acid production by biofilms on composite containing 3% DMADDM was reduced to only 1% of that on composite control. Biofilm colony-forming unit (CFU) counts on composite with 3% DMADDM were reduced by 2-3 orders of magnitude. SIGNIFICANCE: New antibacterial monomers were synthesized, and the carbon chain length had a strong effect on antibacterial efficacy. The new DMADDM-NACP nanocomposite possessed potent anti-biofilm activity without compromising load-bearing properties, and is promising for antibacterial and remineralizing dental restorations to inhibit secondary caries.
Authors: Solomon Praveen Samuel; Shuxi Li; Indraneil Mukherjee; Yi Guo; Alpa C Patel; George Baran; Yen Wei Journal: Dent Mater Date: 2008-09-19 Impact factor: 5.304
Authors: Lei Cheng; Ke Zhang; Michael D Weir; Mary Anne S Melo; Xuedong Zhou; Hockin H K Xu Journal: Nanomedicine (Lond) Date: 2015-03 Impact factor: 5.307
Authors: Haohao Wang; Suping Wang; Lei Cheng; Yaling Jiang; Mary Anne S Melo; Michael D Weir; Thomas W Oates; Xuedong Zhou; Hockin H K Xu Journal: Mater Sci Eng C Mater Biol Appl Date: 2018-10-02 Impact factor: 7.328
Authors: Junling Wu; Michael D Weir; Qiang Zhang; Chuanjian Zhou; Mary Anne S Melo; Hockin H K Xu Journal: Dent Mater Date: 2015-12-29 Impact factor: 5.304