Literature DB >> 23765285

Backbone and side-chain assignments of an effector membrane localization domain from Vibrio vulnificus MARTX toxin.

Michael C Brothers1, Brett Geissler, Grant S Hisao, Brenda A Wilson, Karla J F Satchell, Chad M Rienstra.   

Abstract

(1)H, (13)C, and (15)N chemical shift assignments are presented for the isolated four-helical bundle membrane localization domain from the domain of unknown function 5 (DUF5) effector (MLD(VvDUF5)) of the MARTX toxin from Vibrio vulnificus in its solution state. We have assigned 97% of all backbone and side-chain carbon atoms, including 96% of all backbone residues. Secondary chemical shift analysis using TALOS+ demonstrates four helices that align with those predicted by structure homology modeling using the MLDs of Pasteurella multocida toxin (PMT) and the clostridial TcdB and TcsL toxins as templates. Future studies will be towards solving the structure and determining the dynamics in the solution state.

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Year:  2013        PMID： 23765285      PMCID： PMC3859858          DOI： 10.1007/s12104-013-9488-0

Source DB:  PubMed          Journal:  Biomol NMR Assign        ISSN： 1874-270X            Impact factor:   0.731


  11 in total

1.  Characterization of the membrane-targeting C1 domain in Pasteurella multocida toxin.

Authors:  Shigeki Kamitani; Kengo Kitadokoro; Masayuki Miyazawa; Hirono Toshima; Aya Fukui; Hiroyuki Abe; Masami Miyake; Yasuhiko Horiguchi
Journal:  J Biol Chem       Date:  2010-06-09       Impact factor: 5.157

Review 2.  Structure and function of MARTX toxins and other large repetitive RTX proteins.

Authors:  Karla J F Satchell
Journal:  Annu Rev Microbiol       Date:  2011       Impact factor: 15.500

3.  Identification of a conserved membrane localization domain within numerous large bacterial protein toxins.

Authors:  Brett Geissler; Rehman Tungekar; Karla J F Satchell
Journal:  Proc Natl Acad Sci U S A       Date:  2010-03-08       Impact factor: 11.205

4.  NMRPipe: a multidimensional spectral processing system based on UNIX pipes.

Authors:  F Delaglio; S Grzesiek; G W Vuister; G Zhu; J Pfeifer; A Bax
Journal:  J Biomol NMR       Date:  1995-11       Impact factor: 2.835

5.  Overcoming the overlap problem in the assignment of 1H NMR spectra of larger proteins by use of three-dimensional heteronuclear 1H-15N Hartmann-Hahn-multiple quantum coherence and nuclear Overhauser-multiple quantum coherence spectroscopy: application to interleukin 1 beta.

Authors:  D Marion; P C Driscoll; L E Kay; P T Wingfield; A Bax; A M Gronenborn; G M Clore
Journal:  Biochemistry       Date:  1989-07-25       Impact factor: 3.162

6.  Plasma membrane association of three classes of bacterial toxins is mediated by a basic-hydrophobic motif.

Authors:  Brett Geissler; Sebastian Ahrens; Karla J F Satchell
Journal:  Cell Microbiol       Date:  2011-11-29       Impact factor: 3.715

7.  Protein production by auto-induction in high density shaking cultures.

Authors:  F William Studier
Journal:  Protein Expr Purif       Date:  2005-05       Impact factor: 1.650

Review 8.  Membrane lipids: where they are and how they behave.

Authors:  Gerrit van Meer; Dennis R Voelker; Gerald W Feigenson
Journal:  Nat Rev Mol Cell Biol       Date:  2008-02       Impact factor: 94.444

9.  Crystal structures reveal a thiol protease-like catalytic triad in the C-terminal region of Pasteurella multocida toxin.

Authors:  Kengo Kitadokoro; Shigeki Kamitani; Masayuki Miyazawa; Miyuki Hanajima-Ozawa; Aya Fukui; Masami Miyake; Yasuhiko Horiguchi
Journal:  Proc Natl Acad Sci U S A       Date:  2007-03-14       Impact factor: 11.205

10.  TALOS+: a hybrid method for predicting protein backbone torsion angles from NMR chemical shifts.

Authors:  Yang Shen; Frank Delaglio; Gabriel Cornilescu; Ad Bax
Journal:  J Biomol NMR       Date:  2009-06-23       Impact factor: 2.835
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  4 in total

1.  The membrane localization domains of two distinct bacterial toxins form a 4-helix-bundle in solution.

Authors:  Grant S Hisao; Michael C Brothers; Mengfei Ho; Brenda A Wilson; Chad M Rienstra
Journal:  Protein Sci       Date:  2017-02-14       Impact factor: 6.725

2.  Cytotoxicity of the Vibrio vulnificus MARTX toxin effector DUF5 is linked to the C2A subdomain.

Authors:  Irena Antic; Marco Biancucci; Karla J F Satchell
Journal:  Proteins       Date:  2014-06-26

3.  The Tip of the Four N-Terminal α-Helices of Clostridium sordellii Lethal Toxin Contains the Interaction Site with Membrane Phosphatidylserine Facilitating Small GTPases Glucosylation.

Authors:  Carolina Varela Chavez; Georges Michel Haustant; Bruno Baron; Patrick England; Alexandre Chenal; Serge Pauillac; Arnaud Blondel; Michel-Robert Popoff
Journal:  Toxins (Basel)       Date:  2016-03-25       Impact factor: 4.546

4.  Site-specific processing of Ras and Rap1 Switch I by a MARTX toxin effector domain.

Authors:  Irena Antic; Marco Biancucci; Yueming Zhu; David R Gius; Karla J F Satchell
Journal:  Nat Commun       Date:  2015-06-08       Impact factor: 17.694
  4 in total

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