BACKGROUND: Scedosporium and Pseudallescheria species are the second most common lung-colonising fungi in cystic fibrosis (CF) patients. For epidemiological reasons it is important to trace sources of infection, routes of transmission and to determine whether these fungi are transient or permanent colonisers of the respiratory tract. Molecular typing methods like multilocus sequence typing (MLST) help provide this data. METHODS: Clinical isolates of the P. boydii complex (including S. apiospermum and P. boydii) from CF patients in different regions of Germany were studied using MLST. Five gene loci, ACT, CAL, RPB2, BT2 and SOD2, were analysed. RESULTS: The S. apiospermum isolates from 34 patients were assigned to 32 sequence types (STs), and the P. boydii isolates from 14 patients to 8 STs. The results revealed that patients can be colonised by individual strains for years. CONCLUSIONS: The MLST scheme developed for S. apiospermum and P. boydii is a highly effective tool for epidemiologic studies worldwide. The MLST data are accessible at http://mlst.mycologylab.org/.
BACKGROUND:Scedosporium and Pseudallescheria species are the second most common lung-colonising fungi in cystic fibrosis (CF) patients. For epidemiological reasons it is important to trace sources of infection, routes of transmission and to determine whether these fungi are transient or permanent colonisers of the respiratory tract. Molecular typing methods like multilocus sequence typing (MLST) help provide this data. METHODS: Clinical isolates of the P. boydii complex (including S. apiospermum and P. boydii) from CFpatients in different regions of Germany were studied using MLST. Five gene loci, ACT, CAL, RPB2, BT2 and SOD2, were analysed. RESULTS: The S. apiospermum isolates from 34 patients were assigned to 32 sequence types (STs), and the P. boydii isolates from 14 patients to 8 STs. The results revealed that patients can be colonised by individual strains for years. CONCLUSIONS: The MLST scheme developed for S. apiospermum and P. boydii is a highly effective tool for epidemiologic studies worldwide. The MLST data are accessible at http://mlst.mycologylab.org/.
Authors: Yuan Chen; Aubrey E Frazzitta; Anastasia P Litvintseva; Charles Fang; Thomas G Mitchell; Deborah J Springer; Yun Ding; George Yuan; John R Perfect Journal: Fungal Genet Biol Date: 2015-01-24 Impact factor: 3.495
Authors: M Lackner; F Hagen; J F Meis; A H G Gerrits van den Ende; D Vu; V Robert; J Fritz; T A A Moussa; G S de Hoog Journal: Antimicrob Agents Chemother Date: 2014-07-28 Impact factor: 5.191
Authors: Sven D Willger; Sharon L Grim; Emily L Dolben; Anna Shipunova; Thomas H Hampton; Hilary G Morrison; Laura M Filkins; George A O'Toole; Lisa A Moulton; Alix Ashare; Mitchell L Sogin; Deborah A Hogan Journal: Microbiome Date: 2014-11-03 Impact factor: 14.650
Authors: S Seyedmousavi; Y C Chang; J H Youn; D Law; M Birch; J H Rex; K J Kwon-Chung Journal: Antimicrob Agents Chemother Date: 2021-07-12 Impact factor: 5.191