OBJECTIVE: The objective of the study was to determine if zinc-doped etch-and-rinse dentin adhesive may induce therapeutic effects within the resin-dentin interface. METHODS: Human acid-etched dentin was infiltrated with Adper™ Single Bond Plus (SB, 3M ESPE, St. Paul, MN, USA), SB doped with 10wt.% ZnO nanoparticles (ZnO-SB) or SB doped with 2wt.% ZnCl2 (ZnCl2-SB). AFM/nanoindentation analysis was performed on fully hydrated specimens to evaluate the nanomechanical properties (Hi: hardness; Ei: modulus of elasticity) across the resin-dentin interface after different SBF storage periods (24h, 1m, 3m). Confocal laser microscopy (CLSM) was used to evaluate the ultramorphology and micropermeability at 24h and 3m of SBF storage. RESULTS: SB control specimens exhibited a decrease in Hi in the hybrid layer (HL) and bottom of the hybrid layer (BHL) and a decrease in Ei in the HL after 3m of SBF storage, indicating that severe degradation occurred in the control interface. ZnO-SB bonded specimens preserved the initial Hi and Ei at the HL and BHL subsequent SBF storage; ZnCl2-SB bonded specimens showed a decrease in Ei, in the HL over time. CLSM analysis confirmed that both Zn-doped adhesives were able to preserve the integrity of the HL. SIGNIFICANCE: Specific formulation of Zn-doped etch-and-rinse adhesives may offer the possibility to maintain the nano-mechanical properties along the dentin-bonded interface by inhibiting dentin MMPs and by protective mineral crystals formation within the resin-dentin interface. Clinical advantages may be expected by preserving and improving the integrity of the hybrid layer when Zn-doped adhesives are employed.
OBJECTIVE: The objective of the study was to determine if zinc-doped etch-and-rinse dentin adhesive may induce therapeutic effects within the resin-dentin interface. METHODS:Human acid-etched dentin was infiltrated with Adper™ Single Bond Plus (SB, 3M ESPE, St. Paul, MN, USA), SBdoped with 10wt.% ZnO nanoparticles (ZnO-SB) or SBdoped with 2wt.% ZnCl2 (ZnCl2-SB). AFM/nanoindentation analysis was performed on fully hydrated specimens to evaluate the nanomechanical properties (Hi: hardness; Ei: modulus of elasticity) across the resin-dentin interface after different SBF storage periods (24h, 1m, 3m). Confocal laser microscopy (CLSM) was used to evaluate the ultramorphology and micropermeability at 24h and 3m of SBF storage. RESULTS:SB control specimens exhibited a decrease in Hi in the hybrid layer (HL) and bottom of the hybrid layer (BHL) and a decrease in Ei in the HL after 3m of SBF storage, indicating that severe degradation occurred in the control interface. ZnO-SB bonded specimens preserved the initial Hi and Ei at the HL and BHL subsequent SBF storage; ZnCl2-SB bonded specimens showed a decrease in Ei, in the HL over time. CLSM analysis confirmed that both Zn-doped adhesives were able to preserve the integrity of the HL. SIGNIFICANCE: Specific formulation of Zn-doped etch-and-rinse adhesives may offer the possibility to maintain the nano-mechanical properties along the dentin-bonded interface by inhibiting dentin MMPs and by protective mineral crystals formation within the resin-dentin interface. Clinical advantages may be expected by preserving and improving the integrity of the hybrid layer when Zn-doped adhesives are employed.
Authors: Manuel Toledano; Esther Muñoz-Soto; Fátima S Aguilera; Estrella Osorio; Mayra C Pérez-Álvarez; José Ad García-Menocal; Manuel Toledano-Osorio; Raquel Osorio Journal: Clin Oral Investig Date: 2019-05-17 Impact factor: 3.573
Authors: Haohao Wang; Suping Wang; Lei Cheng; Yaling Jiang; Mary Anne S Melo; Michael D Weir; Thomas W Oates; Xuedong Zhou; Hockin H K Xu Journal: Mater Sci Eng C Mater Biol Appl Date: 2018-10-02 Impact factor: 7.328