Response of the photosynthetic bacterium Rhodobacter sphaeroides to iron limitation and the role of a Fur orthologue in this response.

We studied the response of the photosynthetic alpha-proteobacterium Rhodobacter sphaeroides to iron limitation in order to get first insights into the underlying mechanisms and the link between iron metabolism and oxidative stress. Our data reveal the production of elevated levels of reactive oxygen species upon iron limitation, nevertheless the response to iron limitation shows clear differences to the oxidative stress response of R. sphaeroides. While most genes of the oxidative stress response were not induced by iron limitation, we observed an upregulation of the alternative sigma factor RpoE, which has a main role in the regulation of the defence to singlet oxygen. Deletion of the Fur orthologue RSP_2494, which was designated Mur as a result of a proposed regulatory role in manganese metabolism, revealed that this protein is involved in regulation of the iron metabolism in R. sphaeroides. One predicted target of Fur/Mur is the sit operon encoding a Mn(2+) /Fe(2+) transport system. The basal level of sitA was higher in a fur/mur deletion strain compared with the wild type, which is in agreement with a repressor function of the Fur/Mur protein. In addition, we could also demonstrate a function of the Fur/Mur protein in manganese homeostasis.