| Literature DB >> 237561 |
H Okazaki, M E Ittel, C Niedergang, P Mandel.
Abstract
A ribonuclease (ribonucleate 3-pyrimidine-oligonucleotidohydrolase, EC 3.1.4.22) was purified 8300-fold from soluble fraction of beef brain and its properties were investigated. The enzyme is an endonuclease capable of hydrolyzing tRNA, rRNA, poly(C), but shows no activity towards poly(U), poly(A), and poly(G). The preparation is free of deoxyribonuclease, non-specific phosphodiesterase and phosphomonoesterase activity. The enzyme has a pH optimum of 7.6, is not heat stable, has a molecular weight of 25 000, and has a K-m of 134 mu rRNA and K-m of 1600 mug poly(C) per ml.Entities:
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Year: 1975 PMID： 237561 DOI： 10.1016/0005-2744(75)90155-2
Source DB: PubMed Journal: Biochim Biophys Acta ISSN： 0006-3002