Masafumi Katayama1, Kenichiro Donai1, Hiroyuki Sakakibara2, Yukiko Ohtomo1, Makoto Miyagawa3, Kengo Kuroda1, Hiroko Kodama4, Kazufumi Suzuki5, Noriyuki Kasai6, Katsuhiko Nishimori1, Takafumi Uchida1, Kouichi Watanabe1, Hisashi Aso1, Emiko Isogai1, Hideko Sone7, Tomokazu Fukuda8. 1. Tohoku University, Graduate School of Agricultural Science, 1-1 Tsutsumidori, Amamiyamachi, Aoba-ku, Sendai 981-8555, Japan. 2. Faculty of Agriculture, University of Miyazaki, Japan. 3. Central Experimental Animal Center, Teikyo University School of Medicine, Japan. 4. Department of Health Dietetics, Faculty of Health and Medical Sciences, Teikyo Heisei University, Japan. 5. Institute for Animal Reproduction, Japan. 6. Institute of Animal Experimentation, Tohoku University, Graduate School of Medicine, Japan. 7. National Institute for Environmental Studies, Center for Environmental Risk Research, Japan. 8. Tohoku University, Graduate School of Agricultural Science, 1-1 Tsutsumidori, Amamiyamachi, Aoba-ku, Sendai 981-8555, Japan. Electronic address: tomofukuda@bios.tohoku.ac.jp.
Abstract
BACKGROUND AND AIMS: Large-scale epidemiological studies have shown that drinking more than two cups of coffee per day reduces the risks of hepatitis and liver cancer. However, the heterogeneity of the human genome requires studies of experimental animal models with defined genetic backgrounds to evaluate the coffee effects on liver diseases. We evaluated the efficacy of coffee consumption with one of experimental animal models for human disease. METHOD: We used the Long Evans Cinnamon (LEC) rat, which onsets severe hepatitis and high incidence of liver cancer, due to the accumulation of copper and iron in livers caused by the genetic mutation in Atp7B gene, and leading to the continuous oxidative stress. We determined the expression of inflammation related genes, and amounts of copper and iron in livers, and incidence of the pre-neoplastic foci in the liver tissue of LEC rats. RESULTS: Coffee administration for 25 weeks delayed the occurrence of hepatitis by two weeks, significantly improved survival, reduced the expression of inflammatory cytokines, and reduced the incidence of small pre-neoplastic liver foci in LEC rats. There was no significant difference in the accumulation of copper and iron in livers, indicating that coffee administration does not affect to the metabolism of these metals. These findings indicate that drinking coffee potentially prevents hepatitis and liver carcinogenesis through its anti-inflammatory effects. CONCLUSION: This study showed the efficacy of coffee in the prevention of hepatitis and liver carcinogenesis in the LEC model.
BACKGROUND AND AIMS: Large-scale epidemiological studies have shown that drinking more than two cups of coffee per day reduces the risks of hepatitis and liver cancer. However, the heterogeneity of the human genome requires studies of experimental animal models with defined genetic backgrounds to evaluate the coffee effects on liver diseases. We evaluated the efficacy of coffee consumption with one of experimental animal models for human disease. METHOD: We used the Long Evans Cinnamon (LEC) rat, which onsets severe hepatitis and high incidence of liver cancer, due to the accumulation of copper and iron in livers caused by the genetic mutation in Atp7B gene, and leading to the continuous oxidative stress. We determined the expression of inflammation related genes, and amounts of copper and iron in livers, and incidence of the pre-neoplastic foci in the liver tissue of LEC rats. RESULTS: Coffee administration for 25 weeks delayed the occurrence of hepatitis by two weeks, significantly improved survival, reduced the expression of inflammatory cytokines, and reduced the incidence of small pre-neoplastic liver foci in LEC rats. There was no significant difference in the accumulation of copper and iron in livers, indicating that coffee administration does not affect to the metabolism of these metals. These findings indicate that drinking coffee potentially prevents hepatitis and liver carcinogenesis through its anti-inflammatory effects. CONCLUSION: This study showed the efficacy of coffee in the prevention of hepatitis and liver carcinogenesis in the LEC model.
Authors: Nouf M Al-Rasheed; Hala A Attia; Raeesa A Mohamad; Nawal M Al-Rasheed; Maha A Al-Amin; Asma Al-Onazi Journal: Evid Based Complement Alternat Med Date: 2015-04-06 Impact factor: 2.629
Authors: Christiane Barthel; Sandra Wiegand; Sylvie Scharl; Michael Scharl; Pascal Frei; Stephan R Vavricka; Michael Fried; Michael Christian Sulz; Nico Wiegand; Gerhard Rogler; Luc Biedermann Journal: Nutr J Date: 2015-08-12 Impact factor: 3.271