Literature DB >> 23749298

Whole-rat conditional gene knockout via genome editing.

Andrew J Brown1, Daniel A Fisher, Evguenia Kouranova, Aaron McCoy, Kevin Forbes, Yumei Wu, Rachel Henry, Diana Ji, Andre Chambers, Joe Warren, Weiguo Shu, Edward J Weinstein, Xiaoxia Cui.   

Abstract

Animal models with genetic modifications under temporal and/or spatial control are invaluable to functional genomics and medical research. Here we report the generation of tissue-specific knockout rats via microinjection of zinc-finger nucleases (ZFNs) into fertilized eggs. We generated rats with loxP-flanked (floxed) alleles and a tyrosine hydroxylase promoter-driven cre allele and demonstrated Cre-dependent gene disruption in vivo. Pronuclear microinjection of ZFNs, shown by our data to be an efficient and rapid method for creating conditional knockout rats, should also be applicable in other species.

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Year:  2013        PMID： 23749298     DOI： 10.1038/nmeth.2516

Source DB:  PubMed          Journal:  Nat Methods        ISSN： 1548-7091            Impact factor:   28.547


  16 in total

1.  Knockout rats generated by embryo microinjection of TALENs.

Authors:  Laurent Tesson; Claire Usal; Séverine Ménoret; Elo Leung; Brett J Niles; Séverine Remy; Yolanda Santiago; Anna I Vincent; Xiangdong Meng; Lei Zhang; Philip D Gregory; Ignacio Anegon; Gregory J Cost
Journal:  Nat Biotechnol       Date:  2011-08-05       Impact factor: 54.908

2.  A resource of Cre driver lines for genetic targeting of GABAergic neurons in cerebral cortex.

Authors:  Hiroki Taniguchi; Miao He; Priscilla Wu; Sangyong Kim; Raehum Paik; Ken Sugino; Duda Kvitsiani; Duda Kvitsani; Yu Fu; Jiangteng Lu; Ying Lin; Goichi Miyoshi; Yasuyuki Shima; Gord Fishell; Sacha B Nelson; Z Josh Huang
Journal:  Neuron       Date:  2011-09-21       Impact factor: 17.173

Review 3.  Current issues in mouse genome engineering.

Authors:  Stefan Glaser; Konstantinos Anastassiadis; A Francis Stewart
Journal:  Nat Genet       Date:  2005-11       Impact factor: 38.330

4.  Targeted disruption of NMDA receptor 1 gene abolishes NMDA response and results in neonatal death.

Authors:  D Forrest; M Yuzaki; H D Soares; L Ng; D C Luk; M Sheng; C L Stewart; J I Morgan; J A Connor; T Curran
Journal:  Neuron       Date:  1994-08       Impact factor: 17.173

5.  Engineering a mouse balancer chromosome.

Authors:  B Zheng; M Sage; W W Cai; D M Thompson; B C Tavsanli; Y C Cheah; A Bradley
Journal:  Nat Genet       Date:  1999-08       Impact factor: 38.330

6.  Recombinase-driver rat lines: tools, techniques, and optogenetic application to dopamine-mediated reinforcement.

Authors:  Ilana B Witten; Elizabeth E Steinberg; Soo Yeun Lee; Thomas J Davidson; Kelly A Zalocusky; Matthew Brodsky; Ofer Yizhar; Saemi L Cho; Shiaoching Gong; Charu Ramakrishnan; Garret D Stuber; Kay M Tye; Patricia H Janak; Karl Deisseroth
Journal:  Neuron       Date:  2011-12-08       Impact factor: 17.173

Review 7.  Dopaminergic neurons.

Authors:  Shankar J Chinta; Julie K Andersen
Journal:  Int J Biochem Cell Biol       Date:  2004-12-02       Impact factor: 5.085

8.  Targeted disruption of the tyrosine hydroxylase gene reveals that catecholamines are required for mouse fetal development.

Authors:  Q Y Zhou; C J Quaife; R D Palmiter
Journal:  Nature       Date:  1995-04-13       Impact factor: 49.962

9.  Inducible gene manipulations in brain serotonergic neurons of transgenic rats.

Authors:  Tillmann Weber; Kai Schönig; Björn Tews; Dusan Bartsch
Journal:  PLoS One       Date:  2011-11-29       Impact factor: 3.240

10.  Creation and preliminary characterization of a Tp53 knockout rat.

Authors:  Aaron McCoy; Cynthia L Besch-Williford; Craig L Franklin; Edward J Weinstein; Xiaoxia Cui
Journal:  Dis Model Mech       Date:  2012-08-23       Impact factor: 5.758
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  39 in total

Review 1.  Genome editing revolutionize the creation of genetically modified pigs for modeling human diseases.

Authors:  Jing Yao; Jiaojiao Huang; Jianguo Zhao
Journal:  Hum Genet       Date:  2016-07-18       Impact factor: 4.132

2.  One-step generation of mice carrying reporter and conditional alleles by CRISPR/Cas-mediated genome engineering.

Authors:  Hui Yang; Haoyi Wang; Chikdu S Shivalila; Albert W Cheng; Linyu Shi; Rudolf Jaenisch
Journal:  Cell       Date:  2013-08-29       Impact factor: 41.582

3.  Technical advances in the generation of transgenic animals and in their applications. Nantes, France, June 7th 2013.

Authors:  Séverine Ménoret; Laurent Tesson; Séverine Rémy; Reynald Thinard; Claire Usal; Laure-Hélène Ouisse; Virginie Thepenier; Ignacio Anegon
Journal:  Transgenic Res       Date:  2013-08-02       Impact factor: 2.788

4.  Heritable gene-targeting with gRNA/Cas9 in rats.

Authors:  Xinli Hu; Nannan Chang; Xuelian Wang; Fengyun Zhou; Xiaohai Zhou; Xiaojun Zhu; Jing-Wei Xiong
Journal:  Cell Res       Date:  2013-10-22       Impact factor: 25.617

5.  Generating rats with conditional alleles using CRISPR/Cas9.

Authors:  Yuanwu Ma; Xu Zhang; Bin Shen; Yingdong Lu; Wei Chen; Jing Ma; Lin Bai; Xingxu Huang; Lianfeng Zhang
Journal:  Cell Res       Date:  2013-12-03       Impact factor: 25.617

6.  CRISPR/Cas-mediated genome editing in the rat via direct injection of one-cell embryos.

Authors:  Yanjiao Shao; Yuting Guan; Liren Wang; Zhongwei Qiu; Meizhen Liu; Yuting Chen; Lijuan Wu; Yongmei Li; Xueyun Ma; Mingyao Liu; Dali Li
Journal:  Nat Protoc       Date:  2014-09-25       Impact factor: 13.491

7.  Generation of Esr1-knockout rats using zinc finger nuclease-mediated genome editing.

Authors:  M A Karim Rumi; Pramod Dhakal; Kaiyu Kubota; Damayanti Chakraborty; Tianhua Lei; Melissa A Larson; Michael W Wolfe; Katherine F Roby; Jay L Vivian; Michael J Soares
Journal:  Endocrinology       Date:  2014-02-07       Impact factor: 4.736

Review 8.  Rare inherited kidney diseases: challenges, opportunities, and perspectives.

Authors:  Olivier Devuyst; Nine V A M Knoers; Giuseppe Remuzzi; Franz Schaefer
Journal:  Lancet       Date:  2014-05-24       Impact factor: 79.321

Review 9.  Genetics-based manipulation of adipose tissue sympathetic innervation.

Authors:  Marie François; Emily Qualls-Creekmore; Hans-Rudolf Berthoud; Heike Münzberg; Sangho Yu
Journal:  Physiol Behav       Date:  2017-08-30

Review 10.  Rat embryonic stem cells create new era in development of genetically manipulated rat models.

Authors:  Kazushi Kawaharada; Masaki Kawamata; Takahiro Ochiya
Journal:  World J Stem Cells       Date:  2015-08-26       Impact factor: 5.326
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