Literature DB >> 23748766

Quantification of major royal jelly protein 1 in fresh royal jelly by indirect enzyme-linked immunosorbent assay.

Kikuji Yamaguchi1, Shaoyu He, Zhengyue Li, Kiyoshi Murata, Nobuyuki Hitomi, Manaho Mozumi, Risa Ariga, Toshiki Enomoto.   

Abstract

Rabbit polyclonal antibody produced by a major royal jelly protein 1 (MRJP1) specific peptide reacted only with a MRJP1. Indirect ELISA with the antibody revealed a MRJP1 level of 4.12-4.67 g/100 g in different company's royal jelly, which almost agreed with that of a hexametric form of MRJP1 (apisin) measured by high performance liquid chromatography. These results suggest that MRJP1 exists mainly as apisin in royal jelly.

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Year:  2013        PMID: 23748766     DOI: 10.1271/bbb.130013

Source DB:  PubMed          Journal:  Biosci Biotechnol Biochem        ISSN: 0916-8451            Impact factor:   2.043


  4 in total

1.  Determination of royal jelly freshness by ELISA with a highly specific anti-apalbumin 1, major royal jelly protein 1 antibody.

Authors:  Li-rong Shen; Yi-ran Wang; Liang Zhai; Wen-xiu Zhou; Liang-liang Tan; Mei-lu Li; Dan-dan Liu; Fa Xiao
Journal:  J Zhejiang Univ Sci B       Date:  2015-02       Impact factor: 3.066

2.  Quantitative Analysis of Apisin, a Major Protein Unique to Royal Jelly.

Authors:  Takako Furusawa; Yasuko Arai; Kenji Kato; Kenji Ichihara
Journal:  Evid Based Complement Alternat Med       Date:  2016-09-18       Impact factor: 2.629

3.  Functional and Proteomic Investigations Reveal Major Royal Jelly Protein 1 Associated with Anti-hypertension Activity in Mouse Vascular Smooth Muscle Cells.

Authors:  Pei Fan; Bin Han; Mao Feng; Yu Fang; Lan Zhang; Han Hu; Yue Hao; Yuping Qi; Xiaozhen Zhang; Jianke Li
Journal:  Sci Rep       Date:  2016-07-22       Impact factor: 4.379

4.  Differences in the morphology, physiology and gene expression of honey bee queens and workers reared in vitro versus in situ.

Authors:  Daiana A De Souza; Osman Kaftanoglu; David De Jong; Robert E Page; Gro V Amdam; Ying Wang
Journal:  Biol Open       Date:  2018-11-22       Impact factor: 2.422

  4 in total

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