| Literature DB >> 23747421 |
Luo Zhang1, Xia Xia, Minghua Zhang, Yiwu Wang, Guichun Xing, Xiushan Yin, Lei Song, Fuchu He, Lingqiang Zhang.
Abstract
Casein kinase-2 interacting protein-1 (CKIP-1) has been identified to play an important role in cell morphology, differentiation and apoptosis. However, the role of CKIP-1 in other cellular processes is still unknown. Here we investigated transcriptome profiles of WT and CKIP-1-deficient mouse embryonic fibroblasts (MEFs), and found that innate immunity and cell migration related pathways were significantly correlated with CKIP-1 expression. As macrophage is a key cell type in innate immunity, we then used murine macrophage RAW264.7 cells to discover CKIP-1 interacting proteins by immunoprecipitation/mass spectrometry (IP/MS). Analysis of these proteins revealed migration related pathways were enriched. Further experiments indicated that knockdown of CKIP-1 in RAW264.7 cells resulted in impaired cell migration. Our study suggests that CKIP-1 is a novel regulator of macrophage migration.Entities:
Keywords: CKIP-1; IP/MS; Macrophage migration; Microarray
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Year: 2013 PMID： 23747421 DOI： 10.1016/j.bbrc.2013.05.109
Source DB: PubMed Journal: Biochem Biophys Res Commun ISSN： 0006-291X Impact factor: 3.575