PURPOSE: Minocycline is known as a chemical with neuroprotective, anti-inflammatory, and antimicrobial properties. In this study, the effects of minocycline on seizures induced by amygdala kindling in rats were studied. METHODS: Kindled Wistar rats were injected intraperitoneally with saline and, on the following day, with minocycline (50, 25, and 12.5mg/kg for the three groups (1-3), respectively). The animals in groups 1-3 had similar protocols. Groups 4 and 5 were given for the rotarod test and received 25 or 50mg/kg minocycline, respectively, without any kindling stimulation. The animals in groups 6 and 7 (seven each) received 25mg/kg minocycline or saline, respectively. All the injections were carried out 1h before kindling stimulation. Seizure parameters, including after discharge duration (ADD), stage 4 latency (S4L), stage 5 duration (S5D), and seizure duration (SD), were recorded and compared with those of the saline groups. RESULTS: Minocycline (50mg/kg) significantly reduced ADD, 1/S4L, S5D, and SD (P<0.001, P<0.05, P<0.001, and P<0.001, respectively) in group 1. While the administration of 25mg/kg of minocycline decreased the ADD and S5D (P<0.05), in group 2. The injection of 12.5mg/kg resulted in decreased S5D (P<0.001) in group 3. The daily injection of minocycline (25mg/kg) significantly decreased ADD, S5D, and SD (P<0.001) in group 6. CONCLUSION: The obtained results revealed that minocycline has anticonvulsant effect on seizures induced by amygdala kindling. Thus, it may be useful for epilepsy treatment.
PURPOSE:Minocycline is known as a chemical with neuroprotective, anti-inflammatory, and antimicrobial properties. In this study, the effects of minocycline on seizures induced by amygdala kindling in rats were studied. METHODS: Kindled Wistar rats were injected intraperitoneally with saline and, on the following day, with minocycline (50, 25, and 12.5mg/kg for the three groups (1-3), respectively). The animals in groups 1-3 had similar protocols. Groups 4 and 5 were given for the rotarod test and received 25 or 50mg/kg minocycline, respectively, without any kindling stimulation. The animals in groups 6 and 7 (seven each) received 25mg/kg minocycline or saline, respectively. All the injections were carried out 1h before kindling stimulation. Seizure parameters, including after discharge duration (ADD), stage 4 latency (S4L), stage 5 duration (S5D), and seizure duration (SD), were recorded and compared with those of the saline groups. RESULTS:Minocycline (50mg/kg) significantly reduced ADD, 1/S4L, S5D, and SD (P<0.001, P<0.05, P<0.001, and P<0.001, respectively) in group 1. While the administration of 25mg/kg of minocycline decreased the ADD and S5D (P<0.05), in group 2. The injection of 12.5mg/kg resulted in decreased S5D (P<0.001) in group 3. The daily injection of minocycline (25mg/kg) significantly decreased ADD, S5D, and SD (P<0.001) in group 6. CONCLUSION: The obtained results revealed that minocycline has anticonvulsant effect on seizures induced by amygdala kindling. Thus, it may be useful for epilepsy treatment.
Authors: Stacey B B Dutton; Karoni Dutt; Ligia A Papale; Sandra Helmers; Alan L Goldin; Andrew Escayg Journal: Exp Neurol Date: 2017-04-01 Impact factor: 5.330
Authors: Diede Wm Broekaart; Alexandra Bertran; Shaobo Jia; Anatoly Korotkov; Oleg Senkov; Anika Bongaarts; James D Mills; Jasper J Anink; Jesús Seco; Johannes C Baayen; Sander Idema; Elodie Chabrol; Albert J Becker; Wytse J Wadman; Teresa Tarragó; Jan A Gorter; Eleonora Aronica; Roger Prades; Alexander Dityatev; Erwin A van Vliet Journal: J Clin Invest Date: 2021-01-04 Impact factor: 14.808