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Literature DB >> 2373996

Oxygen-dependent modulation of erythropoietin mRNA levels in isolated rat kidneys studied by RNase protection.

P J Ratcliffe¹, R W Jones, R E Phillips, L G Nicholls, J I Bell.

Abstract

Using oligonucleotide primers complementary to conserved regions in the mouse erythropoietin (Epo) gene, a portion of the rat Epo gene was amplified by the polymerase chain reaction to produce a probe suitable for assay of rat Epo mRNA by RNAse protection. The assay, which has sufficient sensitivity to measure to Epo mRNA in unstimulated rat kidneys, was used to demonstrate high amplitude in vitro modulation of Epo mRNA levels in response to changes in perfusate flow rate and oxygen tension in isolated kidneys, thus providing clear evidence that all the necessary events linking changes in oxygen delivery to the modulation of Epo mRNA levels can occur intrarenally.

Entities: Chemical Gene Species

Mesh：

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Year: 1990 PMID： 2373996 PMCID： PMC2188346 DOI： 10.1084/jem.172.2.657

Source DB: PubMed Journal: J Exp Med ISSN： 0022-1007 Impact factor: 14.307

8 in total

1. PRODUCTION OF ERYTHROPOIETIN BY ANOXIC PERFUSION OF THE ISOLATED KIDNEY OF A DOG.

Authors: E O ZANGHERI; H CAMPANA; F PONCE; J C SILVA; F O FERNANDEZ; J R SUAREZ
Journal: Nature Date: 1963-08-10 Impact factor: 49.962

2. The production of an erythropoietic factor by the in situ perfused kidney.

Authors: J W FISHER; B J BIRDWELL
Journal: Acta Haematol Date: 1961 Impact factor: 2.195

3. Stimulation of erythropoietin gene transcription during hypoxia and cobalt exposure.

Authors: S J Schuster; E V Badiavas; P Costa-Giomi; R Weinmann; A J Erslev; J Caro
Journal: Blood Date: 1989-01 Impact factor: 22.113

4. Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.

Authors: D A Melton; P A Krieg; M R Rebagliati; T Maniatis; K Zinn; M R Green
Journal: Nucleic Acids Res Date: 1984-09-25 Impact factor: 16.971

Review 5. Renal ischemia: a new perspective.

Authors: M Brezis; S Rosen; P Silva; F H Epstein
Journal: Kidney Int Date: 1984-10 Impact factor: 10.612

6. Regulation of the erythropoietin gene: evidence that the oxygen sensor is a heme protein.

Authors: M A Goldberg; S P Dunning; H F Bunn
Journal: Science Date: 1988-12-09 Impact factor: 47.728

7. Physiologic regulation and tissue localization of renal erythropoietin messenger RNA.

Authors: S J Schuster; J H Wilson; A J Erslev; J Caro
Journal: Blood Date: 1987-07 Impact factor: 22.113

8. Expression of the erythropoietin gene.

Authors: N Beru; J McDonald; C Lacombe; E Goldwasser
Journal: Mol Cell Biol Date: 1986-07 Impact factor: 4.272

8 in total

18 in total

1. German Physiology Society. Abstracts of the Spring Meeting (69th Meeting). 5-8 March 1991, Freiburg.

Authors:
Journal: Pflugers Arch Date: 1991 Impact factor: 3.657

2. Diurnal and light-regulated expression of AtSTP1 in guard cells of Arabidopsis.

Authors: Ruth Stadler; Michael Büttner; Peter Ache; Rainer Hedrich; Natalya Ivashikina; Michael Melzer; Sarah M Shearson; Steven M Smith; Norbert Sauer
Journal: Plant Physiol Date: 2003-08-14 Impact factor: 8.340

3. The sink-specific and stress-regulated Arabidopsis STP4 gene: enhanced expression of a gene encoding a monosaccharide transporter by wounding, elicitors, and pathogen challenge.

Authors: E Truernit; J Schmid; P Epple; J Illig; N Sauer
Journal: Plant Cell Date: 1996-12 Impact factor: 11.277

Oxygen-dependent modulation of erythropoietin mRNA levels in isolated rat kidneys studied by RNase protection.

1. PRODUCTION OF ERYTHROPOIETIN BY ANOXIC PERFUSION OF THE ISOLATED KIDNEY OF A DOG.

2. The production of an erythropoietic factor by the in situ perfused kidney.

3. Stimulation of erythropoietin gene transcription during hypoxia and cobalt exposure.

4. Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.

Review 5. Renal ischemia: a new perspective.

6. Regulation of the erythropoietin gene: evidence that the oxygen sensor is a heme protein.

7. Physiologic regulation and tissue localization of renal erythropoietin messenger RNA.

8. Expression of the erythropoietin gene.

1. German Physiology Society. Abstracts of the Spring Meeting (69th Meeting). 5-8 March 1991, Freiburg.

2. Diurnal and light-regulated expression of AtSTP1 in guard cells of Arabidopsis.

3. The sink-specific and stress-regulated Arabidopsis STP4 gene: enhanced expression of a gene encoding a monosaccharide transporter by wounding, elicitors, and pathogen challenge.

4. Divergent regulation of vascular endothelial growth factor and of erythropoietin gene expression in vivo.

5. William Kaelin, Peter Ratcliffe, and Gregg Semenza receive the 2016 Albert Lasker Basic Medical Research Award.

6. Modulation of erythropoietin formation by changes in blood volume in conscious dogs.

7. Age-dependent expression of the erythropoietin gene in rat liver and kidneys.

8. Organ distribution of the three rat endothelin messenger RNAs and the effects of ischemia on renal gene expression.

9. Influence of dietary NaCl intake on renin gene expression in the kidneys and adrenal glands of rats.

10. Oxygen-dependent expression of the erythropoietin gene in rat hepatocytes in vitro.