Claudia Ouschan1, Alexandra Kuchar, Erich Möstl. 1. Department of Companion Animals and Horses, University of Veterinary Medicine, Veterinärplatz 1, A-1210, Vienna, Austria. ouschanc@gmx.at
Abstract
BACKGROUND: The clinical signs of hyperadrenocorticism (hypercortisolism) in dogs are known to be caused by chronic overexposure to glucocorticoids. The quantification of cortisol in serum, saliva or urine reflects the cortisol concentration at the time of sample collection, but in suspected hyperadrenocorticism it may be preferable to examine a long-term parameter of cortisol production. HYPOTHESIS/ OBJECTIVES: There is a need for a noninvasive method to monitor the long-term production of cortisol in dogs. It seems possible that measuring cortisol levels in hair could represent such a method. ANIMALS: Hair was collected from 12 dogs with hyperadrenocorticism and from 10 healthy control dogs. METHODS: Immunoreactive cortisol, cortisone and corticosterone concentrations were determined by enzyme immunoassay. High-performance liquid chromatography was performed to test the validity of the cortisol assay. RESULTS: Levels of immunoreactive cortisol, cortisone and corticosterone were significantly higher in dogs with hyperadrenocorticism than in control dogs. The difference was most pronounced for the cortisol level. CONCLUSIONS AND CLINICAL IMPORTANCE: The determination of cortisol in hair offers the advantage that sampling is easier and less invasive than taking blood, urine, faeces or saliva. Measuring cortisol in hair may represent a valuable tool for the diagnosis of hyperadrenocorticism in dogs.
BACKGROUND: The clinical signs of hyperadrenocorticism (hypercortisolism) in dogs are known to be caused by chronic overexposure to glucocorticoids. The quantification of cortisol in serum, saliva or urine reflects the cortisol concentration at the time of sample collection, but in suspected hyperadrenocorticism it may be preferable to examine a long-term parameter of cortisol production. HYPOTHESIS/ OBJECTIVES: There is a need for a noninvasive method to monitor the long-term production of cortisol in dogs. It seems possible that measuring cortisol levels in hair could represent such a method. ANIMALS: Hair was collected from 12 dogs with hyperadrenocorticism and from 10 healthy control dogs. METHODS: Immunoreactive cortisol, cortisone and corticosterone concentrations were determined by enzyme immunoassay. High-performance liquid chromatography was performed to test the validity of the cortisol assay. RESULTS: Levels of immunoreactive cortisol, cortisone and corticosterone were significantly higher in dogs with hyperadrenocorticism than in control dogs. The difference was most pronounced for the cortisol level. CONCLUSIONS AND CLINICAL IMPORTANCE: The determination of cortisol in hair offers the advantage that sampling is easier and less invasive than taking blood, urine, faeces or saliva. Measuring cortisol in hair may represent a valuable tool for the diagnosis of hyperadrenocorticism in dogs.
Authors: Debora Groppetti; Sara Meazzi; Joel F S Filipe; Carla Colombani; Sara Panseri; Sergio A Zanzani; Clara Palestrini; Simona Cannas; Alessia Giordano; Alessandro Pecile Journal: PLoS One Date: 2021-07-22 Impact factor: 3.240
Authors: Grace B Bowland; Robin M Bernstein; Jeremy Koster; Christine Fiorello; Maris Brenn-White; James Liu; Laura Schwartz; Amanda Campbell; Devin von Stade; Janet Beagley; Julie Pomerantz; Alejandro González; Mackenzie Quick; Kailyn McKinnon; Andrea Aghaian; Corey Sparks; Joshua B Gross Journal: Front Vet Sci Date: 2020-10-19