Literature DB >> 23735698

How crowded is the prokaryotic cytoplasm?

Jan Spitzer1, Bert Poolman.   

Abstract

We consider biomacromolecular crowding within the cytoplasm of prokaryotic cells as a two-phase system of 'supercrowded' cytogel and 'dilute' cytosol; we simplify and quantify this model for a coccoid cell over a wide range of biomacromolecular crowding. The key result shows that the supercrowded cytogel extends the vectorial character of the plasma membrane deeper into the cytoplasm by about 20-70 nm. We discuss useful physiological insights that this model gives into the functioning of a prokaryotic cell on the micrometer scale.
Copyright © 2013 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

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Year:  2013        PMID: 23735698     DOI: 10.1016/j.febslet.2013.05.051

Source DB:  PubMed          Journal:  FEBS Lett        ISSN: 0014-5793            Impact factor:   4.124


  26 in total

1.  The Influence of Crowding Conditions on the Thermodynamic Feasibility of Metabolic Pathways.

Authors:  Liliana Angeles-Martinez; Constantinos Theodoropoulos
Journal:  Biophys J       Date:  2015-12-01       Impact factor: 4.033

2.  Impact of reconstituted cytosol on protein stability.

Authors:  Mohona Sarkar; Austin E Smith; Gary J Pielak
Journal:  Proc Natl Acad Sci U S A       Date:  2013-11-11       Impact factor: 11.205

3.  Specific ion effects on macromolecular interactions in Escherichia coli extracts.

Authors:  Ciara Kyne; Brian Ruhle; Virginie W Gautier; Peter B Crowley
Journal:  Protein Sci       Date:  2014-12-30       Impact factor: 6.725

Review 4.  Microorganisms maintain crowding homeostasis.

Authors:  Jonas van den Berg; Arnold J Boersma; Bert Poolman
Journal:  Nat Rev Microbiol       Date:  2017-03-27       Impact factor: 60.633

5.  Design and Properties of Genetically Encoded Probes for Sensing Macromolecular Crowding.

Authors:  Boqun Liu; Christoffer Åberg; Floris J van Eerden; Siewert J Marrink; Bert Poolman; Arnold J Boersma
Journal:  Biophys J       Date:  2017-05-09       Impact factor: 4.033

Review 6.  Emergence of Life on Earth: A Physicochemical Jigsaw Puzzle.

Authors:  Jan Spitzer
Journal:  J Mol Evol       Date:  2016-12-19       Impact factor: 2.395

7.  Self-propulsion of droplets driven by an active permeating gel.

Authors:  R Kree; A Zippelius
Journal:  Eur Phys J E Soft Matter       Date:  2018-10-11       Impact factor: 1.890

Review 8.  Engineering of Metabolic Pathways Using Synthetic Enzyme Complexes.

Authors:  Nicholas Smirnoff
Journal:  Plant Physiol       Date:  2018-11-19       Impact factor: 8.340

9.  In Vivo Titration of Folate Pathway Enzymes.

Authors:  Deepika Nambiar; Timkhite-Kulu Berhane; Robert Shew; Bryan Schwarz; Michael R Duff; Elizabeth E Howell
Journal:  Appl Environ Microbiol       Date:  2018-09-17       Impact factor: 4.792

10.  Minimal effects of macromolecular crowding on an intrinsically disordered protein: a small-angle neutron scattering study.

Authors:  David P Goldenberg; Brian Argyle
Journal:  Biophys J       Date:  2014-02-18       Impact factor: 4.033

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