Literature DB >> 23730003

In Vitro Expression and Production of Antibody Against Cymbidium mosaic virus Coat Protein.

A R Sherpa1, V Hallan, A A Zaidi.   

Abstract

Polyclonal rabbit antisera were produced using coat protein of Cymbidium mosaic virus (CymMV) Indian isolate expressed in E. coli as GST fusion. The expressed protein was purified by GST-fusion protein purification kit for use as an immunogen in rabbits. Antisera prepared in this manner reacted in double antibody sandwich enzyme-linked immunosorbent assay (DAS ELISA) with extract from CymMV-infected tissue. The results indicate that polyclonal antisera prepared from expressed CymMV coat proteins were useful for the detection of CymMV in an array of assays. The detection system developed is highly effective for detection of Indian strain of the virus in comparison to kits available in the international market.

Entities:  

Keywords:  CP; CymMV; ELISA; Expression; In vitro; Orchidaceae

Year:  2012        PMID： 23730003      PMCID： PMC3550814          DOI： 10.1007/s13337-012-0069-0

Source DB:  PubMed          Journal:  Indian J Virol        ISSN： 0970-2822


  8 in total

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Journal:  Protein Expr Purif       Date:  2000-06       Impact factor: 1.650

2.  Cloning and sequencing of coat protein gene of an Indian Odontoglossum ringspot virus isolate.

Authors:  A R Sherpa; V Hallan; A A Zaidi
Journal:  Acta Virol       Date:  2004       Impact factor: 1.162

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Authors:  M M Bradford
Journal:  Anal Biochem       Date:  1976-05-07       Impact factor: 3.365

4.  Expression of aggregation-prone recombinant proteins at low temperatures: a comparative study of the Escherichia coli cspA and tac promoter systems.

Authors:  J A Vasina; F Baneyx
Journal:  Protein Expr Purif       Date:  1997-03       Impact factor: 1.650

5.  A Japanese encephalitis virus peptide present on Johnson grass mosaic virus-like particles induces virus-neutralizing antibodies and protects mice against lethal challenge.

Authors:  Manisha Saini; Sudhanshu Vrati
Journal:  J Virol       Date:  2003-03       Impact factor: 5.103

6.  Complete nucleotide sequence analysis of Cymbidium mosaic virus Indian isolate: further evidence for natural recombination among potexviruses.

Authors:  Ang Rinzing Sherpa; Vipin Hallan; Promila Pathak; Aijaz Asghar Zaidi
Journal:  J Biosci       Date:  2007-06       Impact factor: 1.826

7.  Expression of bovine leukemia virus protein p24 in Escherichia coli and its use in the immunoblotting assay.

Authors:  L Bicka; J Kuźmak; B Kozaczyńska; A Plucienniczak; A Skorupska
Journal:  Acta Biochim Pol       Date:  2001       Impact factor: 2.149

8.  Expression of Cardamom mosaic virus coat protein in Escherichia coli and its assembly into filamentous aggregates.

Authors:  Thomas Jacob; R Usha
Journal:  Virus Res       Date:  2002-06       Impact factor: 3.303
  8 in total
  1 in total

1.  Cymbidium Mosaic Virus Infecting Orchids: What, How, and What Next?

Authors:  Mohd Shakir Mohamad Yusop; Zeti-Azura Mohamed-Hussein; Ahmad Bazli Ramzi; Hamidun Bunawan
Journal:  Iran J Biotechnol       Date:  2022-01-01       Impact factor: 1.266
  1 in total

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