Literature DB >> 23729888

Production, Purification of Exo-Polygalacturonase from Soil Isolate Paecilomyces variotii NFCCI 1769 and Its Application.

Nitinkumar P Patil1, Kanchankumar P Patil, Bhushan L Chaudhari, Sudhir B Chincholkar.   

Abstract

The aim of the present study was to produce exo-polygalacturonase from potent soil isolate by submerged fermentation and its application for fruit juice treatment. Pectinase producing strains were selectively isolated from pectin industry waste. A selected isolate C2 was found to produce significant amount of exo-polygalacturonase. The isolate was identified as Paecilomyces variotii on the basis of morphological characteristics and 18S rRNA gene sequence analysis. The exo-polygalacturonase produced by the isolate was purified by ammonium sulphate precipitation, size exclusion chromatography and ion exchange chromatography. The purified enzyme had MW of 39.4 kD based on SDS PAGE. Under partially optimized conditions, purified exo-polygalacturonase showed specific activity of 98.49 U/mg protein at pH 6.0 and 30°C. The enzyme was comparatively stable from 10 to 30°C and the activity decreased with increasing temperature. Purified enzyme brought about considerable reduction in viscosity of fruit juice samples.

Entities:  

Keywords:  Exo-polygalacturonase; Paecilomyces variotii NFCCI 1769; Pectinase

Year:  2011        PMID： 23729888      PMCID： PMC3386462          DOI： 10.1007/s12088-011-0162-x

Source DB:  PubMed          Journal:  Indian J Microbiol        ISSN： 0046-8991            Impact factor:   2.461


  10 in total

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Journal:  Acta Microbiol Pol       Date:  2002

2.  Isolation and properties of pectinases from the fungus Aspergillus japonicus.

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Journal:  Biochemistry (Mosc)       Date:  2003-05       Impact factor: 2.487

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Journal:  Appl Environ Microbiol       Date:  1990-12       Impact factor: 4.792

4.  Production of thermostable pectinase and xylanase for their potential application in bleaching of kraft pulp.

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Journal:  J Ind Microbiol Biotechnol       Date:  2007-08-28       Impact factor: 3.346

5.  Optimization of enzymatic clarification of sapodilla juice: a statistical perspective.

Authors:  Nicemol Jacob; R K Sukumaran; P Prema
Journal:  Appl Biochem Biotechnol       Date:  2008-08-01       Impact factor: 2.926

6.  Endo-xylogalacturonan hydrolase, a novel pectinolytic enzyme.

Authors:  C J van der Vlugt-Bergmans; P J Meeuwsen; A G Voragen; A J van Ooyen
Journal:  Appl Environ Microbiol       Date:  2000-01       Impact factor: 4.792

Review 7.  Heat-resistant fungi of importance to the food and beverage industry.

Authors:  V Tournas
Journal:  Crit Rev Microbiol       Date:  1994       Impact factor: 7.624

Review 8.  Pectins: structure, biosynthesis, and oligogalacturonide-related signaling.

Authors:  B L Ridley; M A O'Neill; D Mohnen
Journal:  Phytochemistry       Date:  2001-07       Impact factor: 4.072

9.  Purification, characterization, kinetic properties, and thermal behavior of extracellular polygalacturonase produced by filamentous fungus Tetracoccosporium sp.

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Journal:  Appl Biochem Biotechnol       Date:  2006-12       Impact factor: 2.926

10.  Purification and partial characterization of an exo-polygalacturonase from Paecilomyces variotii liquid cultures.

Authors:  Andre Ricardo de Lima Damásio; Tony Márcio da Silva; Alexandre Maller; João Atílio Jorge; Hector Francisco Terenzi; Maria de Lourdes Teixeira de Moraes Polizeli
Journal:  Appl Biochem Biotechnol       Date:  2009-05-31       Impact factor: 2.926
  10 in total
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Journal:  Indian J Microbiol       Date:  2013-03-16       Impact factor: 2.461

2.  Purification and characterization of polygalacturonase from Aspergillus fumigatus MTCC 2584 and elucidating its application in retting of Crotalaria juncea fiber.

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Journal:  3 Biotech       Date:  2016-09-22       Impact factor: 2.406

3.  Enhanced production of pectinase by Aspergillus terreus NCFT 4269.10 using banana peels as substrate.

Authors:  Bijay Kumar Sethi; Prativa Kumari Nanda; Santilata Sahoo
Journal:  3 Biotech       Date:  2016-01-23       Impact factor: 2.406

4.  Metabolic engineering of Bacillus subtilis with an endopolygalacturonase gene isolated from Pectobacterium. carotovorum; a plant pathogenic bacterial strain.

Authors:  Nagina Rafique; Saiqa Bashir; Muhammad Zubair Khan; Imran Hayat; Willium Orts; Dominic W S Wong
Journal:  PLoS One       Date:  2021-12-22       Impact factor: 3.240
  4 in total

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