| Literature DB >> 23729382 |
Nanami Shiozaki1, Kentaro Nakano, Yasuharu Kushida, Taro Q P Noguchi, Taro Q P Uyeda, Dorota Wloga, Drashti Dave, Krishna Kumar Vasudevan, Jacek Gaertig, Osamu Numata.
Abstract
ADF/cofilin is a highly conserved actin-modulating protein. Reorganization of the actin cytoskeleton in vivo through severing and depolymerizing of F-actin by this protein is essential for various cellular events, such as endocytosis, phagocytosis, cytokinesis, and cell migration. We show that in the ciliate Tetrahymena thermophila, the ADF/cofilin homologue Adf73p associates with actin on nascent food vacuoles. Overexpression of Adf73p disrupted the proper localization of actin and inhibited the formation of food vacuoles. In vitro, recombinant Adf73p promoted the depolymerization of filaments made of T. thermophila actin (Act1p). Knockout cells lacking the ADF73 gene are viable but grow extremely slowly and have a severely decreased rate of food vacuole formation. Knockout cells have abnormal aggregates of actin in the cytoplasm. Surprisingly, unlike the case in animals and yeasts, in Tetrahymena, ADF/cofilin is not required for cytokinesis. Thus, the Tetrahymena model shows promise for future studies of the role of ADF/cofilin in vivo.Entities:
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Year: 2013 PMID： 23729382 PMCID： PMC3754538 DOI： 10.1128/EC.00074-13
Source DB: PubMed Journal: Eukaryot Cell ISSN： 1535-9786