Guochen Zhu1, Weihua Lou. 1. Department of Otolaryngology, Wuxi Second People's Hospital, Affiliated with Nanjing Medical University, Wuxi, Jiangsu, China.
Abstract
BACKGROUND: Because of ease of harvest and low immunogenicity, xenogeneic acellular nerve graft (XANG) may be an alternative to autologous nerve to repair facial nerve defects. METHODS: Facial nerve defects of Wistar rats were repaired by XANG, and nerve gap regeneration was investigated by electrophysiological test, horseradish peroxidase (HRP) retrograde tracing and histomorphometric analysis, as compared to autograft. RESULTS: Twenty weeks after the grafting, electrophysiology showed that whisker pad muscles responded to the electrical stimuli given at the site proximal to the transplantation in 2 groups. Some HRP-labeled facial motorneurons were located on the facial nucleus of the operated side, and an abundance of myelinated axons were found at the middle of the grafts and obvious motor endplates in the target muscles in 2 groups, although they were inferior to the contralateral side in numbers. CONCLUSION: XANG represents an alternative approach for the reconstruction of peripheral facial nerve defects.
BACKGROUND: Because of ease of harvest and low immunogenicity, xenogeneic acellular nerve graft (XANG) may be an alternative to autologous nerve to repair facial nerve defects. METHODS:Facial nerve defects of Wistar rats were repaired by XANG, and nerve gap regeneration was investigated by electrophysiological test, horseradish peroxidase (HRP) retrograde tracing and histomorphometric analysis, as compared to autograft. RESULTS: Twenty weeks after the grafting, electrophysiology showed that whisker pad muscles responded to the electrical stimuli given at the site proximal to the transplantation in 2 groups. Some HRP-labeled facial motorneurons were located on the facial nucleus of the operated side, and an abundance of myelinated axons were found at the middle of the grafts and obvious motor endplates in the target muscles in 2 groups, although they were inferior to the contralateral side in numbers. CONCLUSION: XANG represents an alternative approach for the reconstruction of peripheral facial nerve defects.
Authors: Fuat Baris Bengur; Conrad Stoy; Mary A Binko; Wayne Vincent Nerone; Caroline Nadia Fedor; Mario G Solari; Kacey G Marra Journal: Tissue Eng Part B Rev Date: 2021-04-13 Impact factor: 7.376