| Literature DB >> 23729255 |
Anthony Mittermaier1, Erick Meneses.
Abstract
Nuclear magnetic resonance (NMR) spectroscopy can provide detailed information on protein-ligand interactions that is inaccessible using other biophysical techniques. This chapter focuses on NMR-based approaches for extracting affinity and rate constants for weakly binding transient protein complexes with lifetimes of less than about a second. Several pulse sequences and analytical techniques are discussed, including line-shape simulations, spin-echo relaxation dispersion methods (CPMG), and magnetization exchange (EXSY) experiments.Mesh：
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Year: 2013 PMID： 23729255 DOI： 10.1007/978-1-62703-398-5_9
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745