Literature DB >> 23728798

Development of a novel FRET probe for the real-time determination of ceramidase activity.

Krishna P Bhabak1, Anett Hauser, Susanne Redmer, Sebastian Banhart, Dagmar Heuer, Christoph Arenz.   

Abstract

Fretful novelty: We developed two novel doubly labelled fluorescent ceramide analogues that exhibit significant FRET and undergo hydrolysis by ceramidases. We present a fluorescent sphingolipid FRET probe that allows homogeneous ratiometric determination of enzyme activity in real-time.
Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

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Year:  2013        PMID： 23728798     DOI： 10.1002/cbic.201300207

Source DB:  PubMed          Journal:  Chembiochem        ISSN： 1439-4227            Impact factor:   3.164


  8 in total

1.  Multiple actions of doxorubicin on the sphingolipid network revealed by flux analysis.

Authors:  Justin M Snider; Magali Trayssac; Christopher J Clarke; Nicholas Schwartz; Ashley J Snider; Lina M Obeid; Chiara Luberto; Yusuf A Hannun
Journal:  J Lipid Res       Date:  2018-12-20       Impact factor: 5.922

2.  Structural Basis for Ceramide Recognition and Hydrolysis by Human Neutral Ceramidase.

Authors:  Michael V Airola; William J Allen; Michael J Pulkoski-Gross; Lina M Obeid; Robert C Rizzo; Yusuf A Hannun
Journal:  Structure       Date:  2015-07-16       Impact factor: 5.006

Review 3.  Approaches for probing and evaluating mammalian sphingolipid metabolism.

Authors:  Justin M Snider; Chiara Luberto; Yusuf A Hannun
Journal:  Anal Biochem       Date:  2019-03-24       Impact factor: 3.365

4.  Activity of neutral and alkaline ceramidases on fluorogenic N-acylated coumarin-containing aminodiols.

Authors:  Mireia Casasampere; Luz Camacho; Francesca Cingolani; Josefina Casas; Meritxell Egido-Gabás; José Luís Abad; Carmen Bedia; Ruijuan Xu; Kai Wang; Daniel Canals; Yusuf A Hannun; Cungui Mao; Gemma Fabrias
Journal:  J Lipid Res       Date:  2015-08-18       Impact factor: 5.922

5.  Improved plaque assay identifies a novel anti-Chlamydia ceramide derivative with altered intracellular localization.

Authors:  Sebastian Banhart; Essa M Saied; Andrea Martini; Sophia Koch; Lukas Aeberhard; Kazimierz Madela; Christoph Arenz; Dagmar Heuer
Journal:  Antimicrob Agents Chemother       Date:  2014-07-07       Impact factor: 5.191

Review 6.  Neutral ceramidase: Advances in mechanisms, cell regulation, and roles in cancer.

Authors:  Nicolas Coant; Yusuf A Hannun
Journal:  Adv Biol Regul       Date:  2018-10-26

7.  Click and count: specific detection of acid ceramidase activity in live cells.

Authors:  Mireia Casasampere; Eduardo Izquierdo; Josefina Casas; José Luís Abad; Xiao Liu; Ruijuan Xu; Cungui Mao; Young-Tae Chang; Antonio Delgado; Gemma Fabrias
Journal:  Chem Sci       Date:  2020-10-22       Impact factor: 9.825

8.  A Novel Visible Range FRET Probe for Monitoring Acid Sphingomyelinase Activity in Living Cells.

Authors:  Christian Kappe; Zainelabdeen H Mohamed; Eyad Naser; Alexander Carpinteiro; Christoph Arenz
Journal:  Chemistry       Date:  2020-04-21       Impact factor: 5.236
  8 in total

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