Determination and evaluation of gas holdup time with the quadratic equation model and comparison with nonlinear models for isothermal gas chromatography.

Gas holdup time (tM) is a basic parameter in isothermal gas chromatography (GC). Determination and evaluation of tM and retention behaviors of n-alkanes under isothermal GC conditions have been extensively studied since the 1950s, but still remains unresolved. The difference equation (DE) model [J. Chromatogr. A 1260: 215-223] reveals retention behaviors of n-alkanes excluding tM, while the quadratic equation (QE) model [J. Chromatogr. A 1260: 224-231] including tM is suitable for applications. In the present study, tM values were calculated with the QE model, which is referred to as tMT, evaluated and compared with other three typical nonlinear models. The QE model gives an accurate estimation of tM in isothermal GC. The tMT values are highly accurate, stable, and easy to calculate and use. There is only one tMT value at each GC condition. The proper classification of tM values can clarify their disagreement and facilitate GC retention data standardization for which tMT values are promising reference tM values.