OBJECTIVE: To investigate the expression level of TUG1 and one of its transcript variants (n377360) in osteosarcoma cells and assess the role of TUG1 in proliferation and apoptosis in the U2OS cell line. METHODS: TUG1 and n377360 expression levels in patients with osteosarcomas and the U2OS human osteosarcoma cell line were evaluated using real-time quantitative PCR. U2OS cells were transected with TUG1 and n377360 siRNA or non-targeting siRNA. MTS was performed to assess the cell proliferation and flow cytometry was applied to analyze apoptosis. RESULTS: We found significantly higher TUG1 and n377360 expression levels in osteosarcoma tissues compared with matched non-tumorous tissues. In line with this, suppression of TUG1 and n377360 expression by siRNA significantly impaired the cell proliferation potential of osteosarcoma cells. Furthermore, inhibition of TUG1 expression significantly promoted osteosarcoma cell apoptosis. CONCLUSIONS: The overexpression of TUG1 and n377360 in osteosarcoma specimens and the functional role of TUG1 and n377360 regarding cell proliferation and apoptosis in an osteosarcoma cell line provided evidence that the use of TUG1 or n377360 may be a viable but an as yet unexplored therapeutic strategy in tumors that over express these factors.
OBJECTIVE: To investigate the expression level of TUG1 and one of its transcript variants (n377360) in osteosarcoma cells and assess the role of TUG1 in proliferation and apoptosis in the U2OS cell line. METHODS:TUG1 and n377360 expression levels in patients with osteosarcomas and the U2OS humanosteosarcoma cell line were evaluated using real-time quantitative PCR. U2OS cells were transected with TUG1 and n377360 siRNA or non-targeting siRNA. MTS was performed to assess the cell proliferation and flow cytometry was applied to analyze apoptosis. RESULTS: We found significantly higher TUG1 and n377360 expression levels in osteosarcoma tissues compared with matched non-tumorous tissues. In line with this, suppression of TUG1 and n377360 expression by siRNA significantly impaired the cell proliferation potential of osteosarcoma cells. Furthermore, inhibition of TUG1 expression significantly promoted osteosarcoma cell apoptosis. CONCLUSIONS: The overexpression of TUG1 and n377360 in osteosarcoma specimens and the functional role of TUG1 and n377360 regarding cell proliferation and apoptosis in an osteosarcoma cell line provided evidence that the use of TUG1 or n377360 may be a viable but an as yet unexplored therapeutic strategy in tumors that over express these factors.
Authors: Chun Hong Xiao; Hai Zhong Yu; Chun Yan Guo; Zhi Mei Wu; Hong Yan Cao; Wei Bing Li; Jian Fen Yuan Journal: Oncol Lett Date: 2018-08-03 Impact factor: 2.967
Authors: Coralee E Tye; Jonathan A R Gordon; Lori A Martin-Buley; Janet L Stein; Jane B Lian; Gary S Stein Journal: J Cell Physiol Date: 2015-03 Impact factor: 6.384
Authors: Robert Iliev; Renata Kleinova; Jaroslav Juracek; Jan Dolezel; Zuzana Ozanova; Michal Fedorko; Dalibor Pacik; Marek Svoboda; Michal Stanik; Ondrej Slaby Journal: Tumour Biol Date: 2016-07-27