| Literature DB >> 23724994 |
Anna M Varizhuk1, Dmitry N Kaluzhny, Roman A Novikov, Alexandr O Chizhov, Igor P Smirnov, Andrey N Chuvilin, Olga N Tatarinova, Gleb Y Fisunov, Galina E Pozmogova, Vladimir L Florentiev.
Abstract
New oligonucleotide analogues with triazole internucleotide linkages were synthesized, and their hybridization properties were studied. The analogues demonstrated DNA binding affinities similar to those of unmodified oligonucleotides. The modification was shown to protect the oligonucleotides from nuclease hydrolysis. The modified oligonucleotides were tested as PCR primers. Modifications remote from the 3'-terminus were tolerated by polymerases. Our results suggest that these new oligonucleotide analogues are among the most promising triazole DNA mimics characterized to date.Entities:
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Year: 2013 PMID： 23724994 DOI： 10.1021/jo400651k
Source DB: PubMed Journal: J Org Chem ISSN： 0022-3263 Impact factor: 4.354