Sabine Reinsch1, Per Ambus. 1. Department of Chemical and Biochemical Engineering, Technical University of Denmark, 2800 Kgs. Lyngby, Denmark. sare@kt.dtu.dk
Abstract
RATIONALE: Pulse-labeling with (13)CO2 and the subsequent analysis of (13)C-carbon via isotope ratio mass spectrometry (IRMS) have been shown to be an excellent method to investigate the terrestrial carbon cycle. Improving (13)CO2 manipulation experiments will facilitate our understanding of carbon cycling processes. METHODS: A mobile field setup for in situ (13)CO2 pulse-labeling was developed for low vegetation field experiments. Two pulse-labeling experiments were conducted in a Danish heathland in September 2010 (Exp1) and May 2011 (Exp2). A flow-through system was developed where labeling chambers were supplied with (13)CO2-enriched air from a gas reservoir. Reservoir and chamber air was sampled over the course of the experiments and analyzed for CO2 concentration and isotopic composition on a GasBench II interfaced with an isotope ratio mass spectrometer. The soil CO2 efflux and the atom% excess in soil respiration were assessed after the (13)CO2-pulse to verify the setup performance. RESULTS: The carbon dioxide concentrations and (13)CO2 enrichments were stable during the experiments. The CO2 concentrations conformed to the aimed values, whereas the (13)CO2 enrichments were lower than expected. The sources of error for the deviation in observed atom% (13)CO2 values are discussed, and a measurement procedure is suggested for samples highly enriched in (13)C by using adjusted resistor settings of the mass spectrometer. However, more work has to be done. Enrichment patterns in soil respiration agree with published observations indicating satisfactory performance of the developed system. CONCLUSIONS: A mobile flow-through system suitable for continuous in situ (13)CO2 pulse-labeling was successfully developed that is easily applicable in remote natural ecosystems.
RATIONALE: Pulse-labeling with (13)CO2 and the subsequent analysis of (13)C-carbon via isotope ratio mass spectrometry (IRMS) have been shown to be an excellent method to investigate the terrestrial carbon cycle. Improving (13)CO2 manipulation experiments will facilitate our understanding of carbon cycling processes. METHODS: A mobile field setup for in situ (13)CO2 pulse-labeling was developed for low vegetation field experiments. Two pulse-labeling experiments were conducted in a Danish heathland in September 2010 (Exp1) and May 2011 (Exp2). A flow-through system was developed where labeling chambers were supplied with (13)CO2-enriched air from a gas reservoir. Reservoir and chamber air was sampled over the course of the experiments and analyzed for CO2 concentration and isotopic composition on a GasBench II interfaced with an isotope ratio mass spectrometer. The soil CO2 efflux and the atom% excess in soil respiration were assessed after the (13)CO2-pulse to verify the setup performance. RESULTS: The carbon dioxide concentrations and (13)CO2 enrichments were stable during the experiments. The CO2 concentrations conformed to the aimed values, whereas the (13)CO2 enrichments were lower than expected. The sources of error for the deviation in observed atom% (13)CO2 values are discussed, and a measurement procedure is suggested for samples highly enriched in (13)C by using adjusted resistor settings of the mass spectrometer. However, more work has to be done. Enrichment patterns in soil respiration agree with published observations indicating satisfactory performance of the developed system. CONCLUSIONS: A mobile flow-through system suitable for continuous in situ (13)CO2 pulse-labeling was successfully developed that is easily applicable in remote natural ecosystems.
Authors: Xiyang Dong; Maik A Jochmann; Martin Elsner; Armin H Meyer; Leonard E Bäcker; Mona Rahmatullah; Daniel Schunk; Guido Lens; Rainer U Meckenstock Journal: Environ Sci Technol Date: 2017-09-27 Impact factor: 9.028