Literature DB >> 2372109

A method for separating bound versus unbound label during radioiodination.

G B Lipford1, Q Feng, G L Wright.   

Abstract

An inexpensive, highly effective, and safe method for the removal of bound versus unbound label during radiolabeling of proteins is described. The technique employs the use of membrane ultrafiltration technology and returns in one step a highly reproducible product of quality superior to that attained by gel chromatography. Advantages of this technique are a reduction in the quantity of liquid and solid radioactive waste and a significant limitation of potentially harmful manipulation and exposure times.

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Year:  1990        PMID: 2372109     DOI: 10.1016/0003-2697(90)90430-h

Source DB:  PubMed          Journal:  Anal Biochem        ISSN: 0003-2697            Impact factor:   3.365


  3 in total

1.  Isolation of Primary Mouse Hepatocytes for Nascent Protein Synthesis Analysis by Non-radioactive L-azidohomoalanine Labeling Method.

Authors:  Esam S B Salem; Kazutoshi Murakami; Toshimasa Takahashi; Elise Bernhard; Vishnupriya Borra; Mridula Bethi; Takahisa Nakamura
Journal:  J Vis Exp       Date:  2018-10-23       Impact factor: 1.355

2.  Interaction of insulin with Pseudomonas pseudomallei.

Authors:  D E Woods; A L Jones; P J Hill
Journal:  Infect Immun       Date:  1993-10       Impact factor: 3.441

3.  Pili and lipopolysaccharide of Pseudomonas aeruginosa bind to the glycolipid asialo GM1.

Authors:  S K Gupta; R S Berk; S Masinick; L D Hazlett
Journal:  Infect Immun       Date:  1994-10       Impact factor: 3.441

  3 in total

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